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PMID: 12119413 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ubiquitin ligase-associated protein SGT1 is required for host and nonhost disease resistance in plants.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 16 ·2002-08-06 ·Pages 10865-9

Peart JR, Lu R, Sadanandom A, Malcuit I, Moffett P, Brice DC, Schauser L, Jaggard DA, Xiao S, Coleman MJ, Dow M, Jones JD, Shirasu K, Baulcombe DC

Abstract

Homologues of the yeast ubiquitin ligase-associated protein SGT1 are required for disease resistance in plants mediated by nucleotide-binding site/leucine-rich repeat (NBS-LRR) proteins. Here, by silencing SGT1 in Nicotiana benthamiana, we extend these findings and demonstrate that SGT1 has an unexpectedly general role in disease resistance. It is required for resistance responses mediated by NBS-LRR and other R proteins in which pathogen-derived elicitors are recognized either inside or outside the host plant cell. A requirement also exists for SGT1 in nonhost resistance in which all known members of a host species are resistant against every characterized isolate of a pathogen. Our findings show that silencing SGT1 affects diverse types of disease resistance in plants and support the idea that R protein-mediated and nonhost resistance may involve similar mechanisms.

MeSH Terms
Amino Acid Sequence Carrier Proteins/genetics,metabolism Gene Silencing Genetic Vectors Ligases/metabolism Molecular Sequence Data Nuclear Proteins/metabolism Plant Diseases Plant Proteins/genetics,metabolism Plant Viruses RNA Viruses Sequence Homology, Amino Acid Tobacco/genetics Ubiquitin-Protein Ligases
Chemicals
Carrier Proteins Nuclear Proteins Plant Proteins R protein, Zea mays Ubiquitin-Protein Ligases Ligases
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Peart Jack R
The Sainsbury Laboratory, John Innes Centre, Colney Lane, Norwich NR4 7UH, United Kingdom.
Lu Rui
Sadanandom Ari
Malcuit Isabelle
Moffett Peter
Brice David C
Schauser Leif
Jaggard Daniel A W
Xiao Shunyuan
Coleman Mark J
Dow Max
Jones Jonathan D G
Shirasu Ken
Baulcombe David C
References (36)
36 references, click to expand
  1. Interactions of the COP9 signalosome with the E3 ubiquitin ligase SCFTIRI in mediating auxin response.
    Science. 2001 May 18;292(5520):1379-82 PMID: 11337587
  2. Characterization of a gene from a tomato pathogen determining hypersensitive resistance in non-host species and genetic analysis of this resistance in bean.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6743-7 PMID: 16593982
  3. Nonhost resistance to Phytophthora: novel prospects for a classical problem.
    Curr Opin Plant Biol. 2001 Aug;4(4):295-300 PMID: 11418338
  4. Cloned avirulence genes from the tomato pathogen Pseudomonas syringae pv. tomato confer cultivar specificity on soybean.
    Proc Natl Acad Sci U S A. 1989 Jan;86(1):157-61 PMID: 16578838
  5. Arabidopsis NHO1 is required for general resistance against Pseudomonas bacteria.
    Plant Cell. 2001 Feb;13(2):437-47 PMID: 11226196
  6. Specific HR-associated recognition of secreted proteins from Cladosporium fulvum occurs in both host and non-host plants.
    Plant J. 2000 Sep;23(6):735-45 PMID: 10998185
  7. Characterization of the Pseudomonas syringae pv. tomato AvrRpt2 protein: demonstration of secretion and processing during bacterial pathogenesis.
    Mol Microbiol. 1999 Jun;32(5):927-41 PMID: 10361296
  8. The Rx gene from potato controls separate virus resistance and cell death responses.
    Plant Cell. 1999 May;11(5):781-92 PMID: 10330465
  9. Identification and molecular mapping of a single Arabidopsis thaliana locus determining resistance to a phytopathogenic Pseudomonas syringae isolate.
    Plant J. 1991 Nov;1(3):289-302 PMID: 21736648
  10. An EDS1 orthologue is required for N-mediated resistance against tobacco mosaic virus.
    Plant J. 2002 Mar;29(5):569-79 PMID: 11874570
  11. The helicase domain of the TMV replicase proteins induces the N-mediated defence response in tobacco.
    Plant J. 1999 Apr;18(1):67-75 PMID: 10341444
  12. Nonhost resistance and nonspecific plant defenses.
    Curr Opin Plant Biol. 2000 Aug;3(4):315-9 PMID: 10873843
  13. Comparison of the hypersensitive response induced by the tomato Cf-4 and Cf-9 genes in Nicotiana spp.
    Mol Plant Microbe Interact. 2000 Apr;13(4):465-9 PMID: 10755310
  14. Broad-spectrum mildew resistance in Arabidopsis thaliana mediated by RPW8.
    Science. 2001 Jan 5;291(5501):118-20 PMID: 11141561
  15. Plants have a sensitive perception system for the most conserved domain of bacterial flagellin.
    Plant J. 1999 May;18(3):265-76 PMID: 10377992
  16. Plant pathogens and integrated defence responses to infection.
    Nature. 2001 Jun 14;411(6839):826-33 PMID: 11459065
  17. Arabidopsis RAR1 exerts rate-limiting control of R gene-mediated defenses against multiple pathogens.
    Plant Cell. 2002 May;14(5):979-92 PMID: 12034891
  18. RAR1 and NDR1 contribute quantitatively to disease resistance in Arabidopsis, and their relative contributions are dependent on the R gene assayed.
    Plant Cell. 2002 May;14(5):1005-15 PMID: 12034893
  19. Resistance of nicotiana benthamiana to phytophthora infestans is mediated by the recognition of the elicitor protein INF1
    Plant Cell. 1998 Sep;10(9):1413-26 PMID: 9724689
  20. Prior exposure to lipopolysaccharide potentiates expression of plant defenses in response to bacteria.
    Plant J. 2002 Feb;29(4):487-95 PMID: 11846881
  21. Fast forward genetics based on virus-induced gene silencing.
    Curr Opin Plant Biol. 1999 Apr;2(2):109-13 PMID: 10322199
  22. The coat protein of potato virus X is a strain-specific elicitor of Rx1-mediated virus resistance in potato.
    Plant J. 1995 Dec;8(6):933-41 PMID: 8580963
  23. THE TOMATO-CLADOSPORIUM FULVUM INTERACTION: A Versatile Experimental System to Study Plant-Pathogen Interactions.
    Annu Rev Phytopathol. 1999;37:335-367 PMID: 11701827
  24. Compromised disease resistance in saponin-deficient plants.
    Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12923-8 PMID: 10536024
  25. SGT1 encodes an essential component of the yeast kinetochore assembly pathway and a novel subunit of the SCF ubiquitin ligase complex.
    Mol Cell. 1999 Jul;4(1):21-33 PMID: 10445024
  26. A novel class of eukaryotic zinc-binding proteins is required for disease resistance signaling in barley and development in C. elegans.
    Cell. 1999 Nov 12;99(4):355-66 PMID: 10571178
  27. Technical Advance. Tobacco rattle virus as a vector for analysis of gene function by silencing.
    Plant J. 2001 Jan;25(2):237-45 PMID: 11169199
  28. Degradation of Aux/IAA proteins is essential for normal auxin signalling.
    Plant J. 2000 Mar;21(6):553-62 PMID: 10758506
  29. The RAR1 interactor SGT1, an essential component of R gene-triggered disease resistance.
    Science. 2002 Mar 15;295(5562):2073-6 PMID: 11847307
  30. Programmed cell death, mitochondria and the plant hypersensitive response.
    Nature. 2001 Jun 14;411(6839):848-53 PMID: 11459068
  31. Arabidopsis SGT1b is required for defense signaling conferred by several downy mildew resistance genes.
    Plant Cell. 2002 May;14(5):993-1003 PMID: 12034892
  32. NDR1, a pathogen-induced component required for Arabidopsis disease resistance.
    Science. 1997 Dec 12;278(5345):1963-5 PMID: 9395402
  33. EDS1, an essential component of R gene-mediated disease resistance in Arabidopsis has homology to eukaryotic lipases.
    Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):3292-7 PMID: 10077677
  34. Regulatory role of SGT1 in early R gene-mediated plant defenses.
    Science. 2002 Mar 15;295(5562):2077-80 PMID: 11847308
  35. Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis.
    Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10306-11 PMID: 9707643
  36. Plant disease resistance triggered by pathogen-derived molecules: refined models of specific recognition.
    Curr Opin Microbiol. 2002 Feb;5(1):44-50 PMID: 11834368
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.152330599
Published
2002-08-06
Epub
2002-00-15
Pages
10865-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC125064
Subset
IM
Databases
GENBANK
AF516180, AF516181
External Links
PubMed source DOI: 10.1073/pnas.152330599 PMC Full Text View journal details
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