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PMID: 12107145 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural and genetic analysis of the BldB protein of Streptomyces coelicolor.

Journal of bacteriology ·Vol. 184 ·No. 15 ·2002-08-00 ·Pages 4270-6

Eccleston M, Ali RA, Seyler R, Westpheling J, Nodwell J

Abstract

We have demonstrated that the bldB gene of Streptomyces coelicolor is required for the formation of aerial hyphae and the synthesis of antibiotics. We also found that BldB forms a higher-order complex (most likely a dimer) and that amino acid residues 20 to 78 are important for this interaction. This region is conserved in the BldB family, suggesting that dimer formation may be a common feature of these proteins.

MeSH Terms

Amino Acid Sequence Amino Acids/genetics,metabolism Anti-Bacterial Agents/biosynthesis Bacterial Proteins/chemistry,genetics Cloning, Molecular Gene Deletion Genome, Bacterial Helix-Turn-Helix Motifs Hyphae/metabolism Molecular Sequence Data Open Reading Frames Phenotype Sequence Alignment Streptomyces/chemistry,genetics,metabolism

Chemicals

Amino Acids Anti-Bacterial Agents Bacterial Proteins BldB protein, Streptomyces coelicolor

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Eccleston Marcus

Antimicrobial Research Centre and Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.

Ali Reem Ahmed

Seyler Richard

Westpheling Janet

Nodwell Justin

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24 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/JB.184.15.4270-4276.2002

Published

2002-08-00

Pages

4270-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC135207

Subset

External Links

PubMed source DOI: 10.1128/JB.184.15.4270-4276.2002 PMC Full Text View journal details

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Structural and genetic analysis of the BldB protein of Streptomyces coelicolor.

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