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PMID: 12107133 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC.

Journal of bacteriology ·Vol. 184 ·No. 15 ·2002-08-00 ·Pages 4161-7

Nagakubo S, Nishino K, Hirata T, Yamaguchi A

Abstract

Overproduction of the response regulator BaeR confers resistance to novobiocin and bile salts in a DeltaacrAB mutant by stimulating drug exporter gene expression. The mdtABC (multidrug transporter ABC, formerly known as yegMNO) genes, which encode a resistance-nodulation-cell division (RND) drug efflux system, are responsible for resistance. The MdtABC system comprises the transmembrane MdtB/MdtC heteromultimer and MdtA membrane fusion protein. MdtAC also confers bile salt, but not novobiocin, resistance. This indicates that the evolution from an MdtC homomultimer to an MdtBC heteromultimer contributed to extend the drug resistance spectrum. A BLAST search suggested that such a heteromultimer-type RND exporter constitutes a unique family among gram-negative organisms.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacterial Proteins/genetics Bile Acids and Salts/pharmacology Carrier Proteins/genetics Drug Resistance, Microbial Drug Resistance, Multiple Escherichia coli/drug effects,genetics Escherichia coli Proteins Gene Deletion Genes, Bacterial/genetics Membrane Proteins/genetics Membrane Transport Proteins Microbial Sensitivity Tests Novobiocin/pharmacology Open Reading Frames/genetics Regulon
Chemicals
Anti-Bacterial Agents Bacterial Proteins Bile Acids and Salts Carrier Proteins Escherichia coli Proteins Mdt-A protein, E coli Membrane Proteins Membrane Transport Proteins Novobiocin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nagakubo Satoshi
Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki-shi, Japan.
Nishino Kunihiko
Hirata Takahiro
Yamaguchi Akihito
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.184.15.4161-4167.2002
Published
2002-08-00
Pages
4161-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC135206
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.184.15.4161-4167.2002 PMC Full Text View journal details
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