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PMID: 12107110 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Eosinophil recruitment in type-2 hypersensitivity pulmonary granulomas: source and contribution of monocyte chemotactic protein-3 (CCL7).

The American journal of pathology ·Vol. 161 ·No. 1 ·2002-07-00 ·Pages 257-66

Shang XZ, Chiu BC, Stolberg V, Lukacs NW, Kunkel SL, Murphy HS, Chensue SW

Abstract

Monocyte chemotactic protein-3 (MCP-3/CCL7) has potent eosinophil chemoattractant properties. The present study determined its relative contribution to the formation of Th2 cytokine-mediated (type-2) eosinophil-rich interstitial lung granulomas induced by antigens of Schistosoma mansoni eggs. Both MCP-3 transcripts and protein levels were more strongly expressed in lungs with type-2 than with type-1 (mycobacterial antigen-elicited Th1-mediated) granulomas. In vivo treatment with neutralizing antibodies demonstrated that MCP-3 abrogated eosinophil accumulation in type-2 lesions by 40 to 50%. Immunohistochemical staining revealed that MCP-3 localized to vessels in or near granulomas suggesting that endothelial cells were an important in situ source of MCP-3. Maximal MCP-3 transcript expression was abrogated by anti-interleukin-4 treatment. Furthermore, cultured mouse lung endothelial cells displayed augmented MCP-3 production in response to interleukin-4. Together, these results suggest that MCP-3 contributes to a significant component of eosinophil recruitment in the type-2 interstitial granuloma formation and Th2 cytokines promote its production.

MeSH Terms
Animals Antibodies/pharmacology Antigens, Helminth/immunology Blood Vessels/metabolism Cell Movement Chemokine CCL7 Cytokines Endothelium, Vascular/metabolism Eosinophils/pathology,physiology Female Granuloma, Respiratory Tract/immunology,pathology,physiopathology Hypersensitivity/immunology Interleukin-4/immunology Lung/metabolism Lung Diseases/immunology,pathology,physiopathology Mice Mice, Inbred CBA Monocyte Chemoattractant Proteins/antagonists & inhibitors,metabolism Pulmonary Circulation Schistosoma mansoni/immunology Th2 Cells/immunology
Chemicals
Antibodies Antigens, Helminth Ccl7 protein, mouse Chemokine CCL7 Cytokines Monocyte Chemoattractant Proteins Interleukin-4
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Shang Xiao-Zhou
Department of Pathology, University of Michigan Hospitals, Ann Arbor, USA.
Chiu Bo-Chin
Stolberg Valerie
Lukacs Nicholas W
Kunkel Steven L
Murphy Hedwig S
Chensue Stephen W
References (40)
40 references, click to expand
  1. Selective recruitment of CCR4-bearing Th2 cells toward antigen-presenting cells by the CC chemokines thymus and activation-regulated chemokine and macrophage-derived chemokine.
    Int Immunol. 1999 Jan;11(1):81-8 PMID: 10050676
  2. Recent advances in chemokines and chemokine receptors.
    Crit Rev Immunol. 1999;19(1):1-47 PMID: 9987599
  3. Depletion of eosinophils in mice through the use of antibodies specific for C-C chemokine receptor 3 (CCR3).
    J Leukoc Biol. 1999 Jun;65(6):846-53 PMID: 10380909
  4. Increased expression of IP-10, IL-8, MCP-1, and MCP-3 in ulcerative colitis.
    Am J Pathol. 1999 Aug;155(2):331-6 PMID: 10433925
  5. Chemokines and chemokine receptors: their role in allergic airway disease.
    J Clin Immunol. 1999 Sep;19(5):250-65 PMID: 10535601
  6. Eosinophil chemotactic chemokines (eotaxin, eotaxin-2, RANTES, monocyte chemoattractant protein-3 (MCP-3), and MCP-4), and C-C chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic (Intrinsic) asthmatics.
    J Immunol. 1999 Dec 1;163(11):6321-9 PMID: 10570327
  7. CC chemokine receptor (CCR)3/eotaxin is followed by CCR4/monocyte-derived chemokine in mediating pulmonary T helper lymphocyte type 2 recruitment after serial antigen challenge in vivo.
    J Exp Med. 2000 Jan 17;191(2):265-74 PMID: 10637271
  8. Chemokines: a new classification system and their role in immunity.
    Immunity. 2000 Feb;12(2):121-7 PMID: 10714678
  9. Interleukin 4 and 13 participation in mycobacterial (type-1) and schistosomal (type-2) antigen-elicited pulmonary granuloma formation: multiparameter analysis of cellular recruitment, chemokine expression and cytokine networks.
    Cytokine. 2000 May;12(5):432-44 PMID: 10857756
  10. Chemokines in inflammation and immunity.
    Immunol Today. 2000 Sep;21(9):418-20 PMID: 10953090
  11. Chemokines induce eosinophil degranulation through CCR-3.
    J Allergy Clin Immunol. 2000 Sep;106(3):507-13 PMID: 10984371
  12. Differential expression of the chemokine receptors by the Th1- and Th2-type effector populations within circulating CD4+ T cells.
    J Leukoc Biol. 2000 Oct;68(4):568-74 PMID: 11037980
  13. Murine eotaxin-2: a constitutive eosinophil chemokine induced by allergen challenge and IL-4 overexpression.
    J Immunol. 2000 Nov 15;165(10):5839-46 PMID: 11067944
  14. Chemokine expression dynamics in mycobacterial (type-1) and schistosomal (type-2) antigen-elicited pulmonary granuloma formation.
    Am J Pathol. 2001 Apr;158(4):1503-15 PMID: 11290568
  15. Eosinophils in asthma and other allergic diseases.
    Br Med Bull. 2000;56(4):985-1003 PMID: 11359633
  16. The C-C chemokine receptors CCR4 and CCR8 identify airway T cells of allergen-challenged atopic asthmatics.
    J Clin Invest. 2001 Jun;107(11):1357-64 PMID: 11390417
  17. Rules of chemokine receptor association with T cell polarization in vivo.
    J Clin Invest. 2001 Nov;108(9):1331-9 PMID: 11696578
  18. Role of IL-4 and IFN-gamma in Schistosoma mansoni egg-induced hypersensitivity granuloma formation. Orchestration, relative contribution, and relationship to macrophage function.
    J Immunol. 1992 Feb 1;148(3):900-6 PMID: 1309844
  19. Anti-IL-4 treatment of Schistosoma mansoni-infected mice inhibits development of T cells and non-B, non-T cells expressing Th2 cytokines while decreasing egg-induced hepatic fibrosis.
    J Immunol. 1994 Jul 15;153(2):753-9 PMID: 8021510
  20. Cytokine function during mycobacterial and schistosomal antigen-induced pulmonary granuloma formation. Local and regional participation of IFN-gamma, IL-10, and TNF.
    J Immunol. 1995 Jun 1;154(11):5969-76 PMID: 7751640
  21. Murine eotaxin: an eosinophil chemoattractant inducible in endothelial cells and in interleukin 4-induced tumor suppression.
    Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8960-4 PMID: 7568052
  22. Expression of a mannose/fucose membrane lectin on human dendritic cells.
    Eur J Immunol. 1996 Feb;26(2):394-400 PMID: 8617309
  23. Identification of a mouse eosinophil receptor for the CC chemokine eotaxin.
    Biochem Biophys Res Commun. 1996 Jun 25;223(3):679-84 PMID: 8687456
  24. Eosinophil recruitment to the lung in a murine model of allergic inflammation. The role of T cells, chemokines, and adhesion receptors.
    J Clin Invest. 1996 Nov 15;98(10):2332-45 PMID: 8941651
  25. Increased expression of mRNA encoding RANTES and MCP-3 in the bronchial mucosa in atopic asthma.
    Eur Respir J. 1996 Dec;9(12):2454-60 PMID: 8980953
  26. Bronchial mucosal expression of the genes encoding chemokines RANTES and MCP-3 in symptomatic atopic and nonatopic asthmatics: relationship to the eosinophil-active cytokines interleukin (IL)-5, granulocyte macrophage-colony-stimulating factor, and IL-3.
    Am J Respir Cell Mol Biol. 1997 Jan;16(1):1-8 PMID: 8998072
  27. Chemokine receptor usage by human eosinophils. The importance of CCR3 demonstrated using an antagonistic monoclonal antibody.
    J Clin Invest. 1997 Jan 15;99(2):178-84 PMID: 9005985
  28. Targeted disruption of the chemokine eotaxin partially reduces antigen-induced tissue eosinophilia.
    J Exp Med. 1997 Feb 17;185(4):785-90 PMID: 9034156
  29. Monocyte chemotactic protein-3 (MCP-3)/fibroblast-induced cytokine (FIC) in eosinophilic inflammation of the airways and the inhibitory effects of an anti-MCP-3/FIC antibody.
    J Immunol. 1997 May 15;158(10):4953-60 PMID: 9144514
  30. Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells.
    Science. 1997 Sep 26;277(5334):2005-7 PMID: 9302298
  31. Mycobacterial and schistosomal antigen-elicited granuloma formation in IFN-gamma and IL-4 knockout mice: analysis of local and regional cytokine and chemokine networks.
    J Immunol. 1997 Oct 1;159(7):3565-73 PMID: 9317156
  32. Expression of monocyte chemotactic protein-3 in human monocytes and endothelial cells.
    Eur Cytokine Netw. 1997 Sep;8(3):271-4 PMID: 9346360
  33. Cloning and functional characterization of a novel human CC chemokine that binds to the CCR3 receptor and activates human eosinophils.
    J Leukoc Biol. 1997 Nov;62(5):667-75 PMID: 9365122
  34. Binding of RANTES, MCP-1, MCP-3, and MIP-1alpha to cells in human skin.
    Am J Pathol. 1998 Mar;152(3):749-57 PMID: 9502417
  35. Flexible programs of chemokine receptor expression on human polarized T helper 1 and 2 lymphocytes.
    J Exp Med. 1998 Mar 16;187(6):875-83 PMID: 9500790
  36. The C-C chemokine receptor CCR3 participates in stimulation of eosinophil arrest on inflammatory endothelium in shear flow.
    J Clin Invest. 1998 May 1;101(9):2017-24 PMID: 9576767
  37. Expression and participation of eotaxin during mycobacterial (type 1) and schistosomal (type 2) antigen-elicited granuloma formation.
    J Immunol. 1998 Oct 15;161(8):4276-82 PMID: 9780203
  38. Heterogeneity of vascular endothelial cells: differences in susceptibility to neutrophil-mediated injury.
    Microvasc Res. 1998 Nov;56(3):203-11 PMID: 9828158
  39. Chemokines and chemokine receptors in T-cell priming and Th1/Th2-mediated responses.
    Immunol Today. 1998 Dec;19(12):568-74 PMID: 9864948
  40. Monocyte chemotactic proteins in allergen-induced inflammation in the nasal mucosa: effect of topical corticosteroids.
    J Allergy Clin Immunol. 1999 Jun;103(6):1036-44 PMID: 10359883
Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/S0002-9440(10)64177-6
Published
2002-07-00
Pages
257-66
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1850678
Subset
IM
Grants
NIAID NIH HHS · N01-AI-55270 · United States
NIAID NIH HHS · R03 AI055270 · United States
NIAID NIH HHS · AI43460 · United States
NIAID NIH HHS · R01 AI043460 · United States
NIAID NIH HHS · R56 AI043460 · United States
External Links
PubMed source DOI: 10.1016/S0002-9440(10)64177-6 PMC Full Text View journal details
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