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PMID: 10433925 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Increased expression of IP-10, IL-8, MCP-1, and MCP-3 in ulcerative colitis.

The American journal of pathology ·Vol. 155 ·No. 2 ·1999-08-00 ·Pages 331-6

Uguccioni M, Gionchetti P, Robbiani DF, Rizzello F, Peruzzo S, Campieri M, Baggiolini M

Abstract

Chemokines are thought to be important for the recruitment of granulocytes and mononuclear cells and thus for the maintenance of inflammation in ulcerative colitis (UC). We have studied the expression of interferon-gamma inducible protein-10 (IP-10), interleukin-8 (IL-8), monocyte chemoattractant protein (MCP)-1, MCP-3, and macrophage inflammatory protein (MIP)-1alpha in UC patients and control individuals to assess the role of these chemokines in disease progression. Colonic biopsies were taken endoscopically from patients and controls, frozen immediately and subsequently stained for IP-10, IL-8, MCP-1, MCP-3, and MIP-1alpha in serial sections. Cells infiltrating the lamina propria but not epithelial cells express the analyzed chemokines. They were differentiated and counted, and chemokine-expressing cells were quantified by image analysis. The percentage of cells expressing IP-10, IL-8, MCP-1, and MCP-3 was significantly enhanced in all UC samples as compared to controls. Expression in the controls was borderline, except for IP-10. No expression of MIP-1alpha was found in controls and UC. IP-10 was also markedly expressed in the mucosa of control biopsies and therefore could have a role in activated T lymphocytes' recruitment into the healthy mucosa.

MeSH Terms
Adult Chemokine CCL2/metabolism Chemokine CCL3 Chemokine CCL4 Chemokine CCL7 Chemokine CXCL10/metabolism Colitis, Ulcerative/metabolism Colon/anatomy & histology Cytokines Female Gene Expression Humans Immunohistochemistry Interleukin-8/metabolism Macrophage Inflammatory Proteins/metabolism Male Middle Aged Monocyte Chemoattractant Proteins/metabolism Up-Regulation
Chemicals
CCL7 protein, human Chemokine CCL2 Chemokine CCL3 Chemokine CCL4 Chemokine CCL7 Chemokine CXCL10 Cytokines Interleukin-8 Macrophage Inflammatory Proteins Monocyte Chemoattractant Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Uguccioni M
Theodor Kocher Institute, University of Bern, Bern, Switzerland.
Gionchetti P
Robbiani D F
Rizzello F
Peruzzo S
Campieri M
Baggiolini M
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/S0002-9440(10)65128-0
Published
1999-08-00
Pages
331-6
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1866859
Subset
IM
External Links
PubMed source DOI: 10.1016/S0002-9440(10)65128-0 PMC Full Text View journal details
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