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PMID: 12097603 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) contains two functional lytic origins of DNA replication.

Journal of virology ·Vol. 76 ·No. 15 ·2002-08-00 ·Pages 7890-6

AuCoin DP, Colletti KS, Xu Y, Cei SA, Pari GS

Abstract

We used a transient-transfection replication assay to identify two functional copies of the human herpesvirus 8 (HHV8) lytic origin of DNA replication (oriLyt). BCLB-1 cells were transfected with HHV8 subgenomic fragments containing the putative lytic origin along with a plasmid expressing viral transactivator open reading frame (ORF) 50. The HHV8 left-end oriLyt (oriLyt-L) lies between ORFs K4.2 and K5 and is composed of a region encoding various transcription factor binding sites and an A+T-rich region and a G+C repeat region. The right-end oriLyt (oriLyt-R) maps between ORF 69 and vFLIP, a region similar to the RRV oriLyt, and is an inverted duplication of oriLyt-L.

MeSH Terms
Cell Line DNA Replication Herpesvirus 8, Human/genetics Immediate-Early Proteins/genetics Plasmids/genetics Replication Origin/genetics,physiology Restriction Mapping Trans-Activators/genetics Transfection Viral Proteins
Chemicals
Immediate-Early Proteins Rta protein, Human herpesvirus 8 Trans-Activators Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
AuCoin David P
Department of Microbiology and Cell and Molecular Biology Program, University of Nevada School of Medicine, Reno, Nevada 89557, USA.
Colletti Kelly S
Xu Yiyang
Cei Sylvia A
Pari Gregory S
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.76.15.7890-7896.2002
Published
2002-08-00
Pages
7890-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC136389
Subset
IM
Grants
NCI NIH HHS · R01 CA085164 · United States
NCI NIH HHS · R01 CA85164 · United States
External Links
PubMed source DOI: 10.1128/jvi.76.15.7890-7896.2002 PMC Full Text View journal details
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