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PMID: 9724796 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A viral gene that activates lytic cycle expression of Kaposi's sarcoma-associated herpesvirus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 18 ·1998-09-01 ·Pages 10866-71

Sun R, Lin SF, Gradoville L, Yuan Y, Zhu F, Miller G

Abstract

Herpesviruses exist in two states, latency and a lytic productive cycle. Here we identify an immediate-early gene encoded by Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus eight (HHV8) that activates lytic cycle gene expression from the latent viral genome. The gene is a homologue of Rta, a transcriptional activator encoded by Epstein-Barr virus (EBV). KSHV/Rta activated KSHV early lytic genes, including virus-encoded interleukin 6 and polyadenylated nuclear RNA, and a late gene, small viral capsid antigen. In cells dually infected with Epstein-Barr virus and KSHV, each Rta activated only autologous lytic cycle genes. Expression of viral cytokines under control of the KSHV/Rta gene is likely to contribute to the pathogenesis of KSHV-associated diseases.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Primers DNA, Complementary Gene Expression Regulation, Neoplastic Genes, Viral Herpesvirus 4, Human/genetics,physiology Herpesvirus 8, Human/genetics,physiology Humans Molecular Sequence Data Sarcoma, Kaposi/virology Sequence Homology, Amino Acid Transcription, Genetic Tumor Cells, Cultured Virus Activation/genetics
Chemicals
DNA Primers DNA, Complementary
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sun R
Departments of Molecular Biophysics and Biochemistry, Genetics, Pediatrics, and Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06520, USA.
Lin S F
Gradoville L
Yuan Y
Zhu F
Miller G
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.18.10866
Published
1998-09-01
Pages
10866-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC27987
Subset
IM
Grants
NCI NIH HHS · T32 CA009159 · United States
NCI NIH HHS · R01 CA070036 · United States
NCI NIH HHS · CA70036 · United States
NCI NIH HHS · R01 CA012055 · United States
NCI NIH HHS · T32CA09159 · United States
NCI NIH HHS · CA12055 · United States
NCI NIH HHS · R37 CA012055 · United States
Databases
GENBANK
U71367, U71368
External Links
PubMed source DOI: 10.1073/pnas.95.18.10866 PMC Full Text View journal details
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