Home LiteratureArticle Details
PMID: 12072506 Published · ppublish English Journal Article

Processing map and essential cleavage sites of the nonstructural polyprotein encoded by ORF1 of the feline calicivirus genome.

Journal of virology ·Vol. 76 ·No. 14 ·2002-07-00 ·Pages 7060-72

Sosnovtsev SV, Garfield M, Green KY

Abstract

Feline calicivirus (FCV) nonstructural proteins are translated as part of a large polyprotein that undergoes autocatalytic processing by the virus-encoded 3C-like proteinase. In this study, we mapped three new cleavage sites (E(46)/A(47), E(331)/D(332), and E(685)/N(686)) recognized by the virus proteinase in the N-terminal part of the open reading frame 1 (ORF1) polyprotein to complete the processing map. Taken together with two sites we identified previously (E(960)/A(961) and E(1071)/S(1072)), the FCV ORF1 polyprotein contains five cleavage sites that define the borders of six proteins with calculated molecular masses of 5.6, 32, 38.9, 30.1, 12.7, and 75.7 kDa, which we designated p5.6, p32, p39 (NTPase), p30, p13 (VPg), and p76 (Pro-Pol), respectively. Mutagenesis of the E to A in each of these cleavage sites in an infectious FCV cDNA clone was lethal for the virus, indicating that these cleavages are essential in a productive virus infection. Mutagenesis of two cleavage sites (E(1345)/T(1346) and E(1419)/G(1420)) within the 75.7-kDa Pro-Pol protein previously mapped in bacterial expression studies was not lethal.

MeSH Terms
3C Viral Proteases Amino Acid Sequence Animals Calicivirus, Feline/enzymology,growth & development,metabolism Cats Cell Line Cysteine Endopeptidases/metabolism Molecular Sequence Data Mutagenesis, Site-Directed Polyproteins/chemistry,genetics,metabolism Protein Biosynthesis Proteins/genetics Transcription, Genetic Viral Nonstructural Proteins/chemistry,genetics,metabolism Viral Proteins/metabolism
Chemicals
Polyproteins Proteins Viral Nonstructural Proteins Viral Proteins Cysteine Endopeptidases 3C Viral Proteases 3C proteases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sosnovtsev Stanislav V
Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-8007, USA. ss216m@nih.gov
Garfield Mark
Green Kim Y
References (39)
39 references, click to expand
  1. Expression and processing of the canine calicivirus capsid precursor.
    J Gen Virol. 2000 Jan;81(Pt 1):195-9 PMID: 10640558
  2. A hidden Markov model for predicting transmembrane helices in protein sequences.
    Proc Int Conf Intell Syst Mol Biol. 1998;6:175-82 PMID: 9783223
  3. Rabbit hemorrhagic disease virus: genome organization and polyprotein processing of a calicivirus studied after transient expression of cDNA constructs.
    Virology. 2000 Oct 25;276(2):349-63 PMID: 11040126
  4. ATP binding and ATPase activities associated with recombinant rabbit hemorrhagic disease virus 2C-like polypeptide.
    J Virol. 2000 Nov;74(22):10846-51 PMID: 11044135
  5. Identification and genomic mapping of the ORF3 and VPg proteins in feline calicivirus virions.
    Virology. 2000 Nov 10;277(1):193-203 PMID: 11062050
  6. Proteinase-polymerase precursor as the active form of feline calicivirus RNA-dependent RNA polymerase.
    J Virol. 2001 Feb;75(3):1211-9 PMID: 11152494
  7. Polypeptide p41 of a Norwalk-like virus is a nucleic acid-independent nucleoside triphosphatase.
    J Virol. 2001 Feb;75(4):1611-9 PMID: 11160659
  8. The genome of hawaii virus and its relationship with other members of the caliciviridae.
    Virus Genes. 2001;23(1):5-16 PMID: 11556401
  9. Infectious respiratory diseases of cats.
    Vet Clin North Am. 1976 Aug;6(3):399-413 PMID: 183336
  10. The location of the polio genome protein in viral RNAs and its implication for RNA synthesis.
    Nature. 1977 Jul 21;268(5617):208-13 PMID: 196204
  11. Membrane fractions active in poliovirus RNA replication contain VPg precursor polypeptides.
    Virology. 1983 Jul 15;128(1):33-47 PMID: 6308897
  12. Multiple sequence alignment with hierarchical clustering.
    Nucleic Acids Res. 1988 Nov 25;16(22):10881-90 PMID: 2849754
  13. A new superfamily of putative NTP-binding domains encoded by genomes of small DNA and RNA viruses.
    FEBS Lett. 1990 Mar 12;262(1):145-8 PMID: 2156730
  14. Nucleotide sequence of a region of the feline calicivirus genome which encodes picornavirus-like RNA-dependent RNA polymerase, cysteine protease and 2C polypeptides.
    Virus Res. 1990 Nov;17(3):145-60 PMID: 2077782
  15. Rabbit hemorrhagic disease virus--molecular cloning and nucleotide sequencing of a calicivirus genome.
    Virology. 1991 Oct;184(2):664-76 PMID: 1840711
  16. The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses.
    J Gen Virol. 1991 Sep;72 ( Pt 9):2197-206 PMID: 1895057
  17. Nucleotide sequence and expression of the capsid protein gene of feline calicivirus.
    J Virol. 1991 Oct;65(10):5440-7 PMID: 1716692
  18. Identification and sequence determination of the capsid protein gene of feline calicivirus.
    Arch Virol. 1992;122(3-4):223-35 PMID: 1731695
  19. Prediction of protein secondary structure at better than 70% accuracy.
    J Mol Biol. 1993 Jul 20;232(2):584-99 PMID: 8345525
  20. Studies of a putative amphipathic helix in the N-terminus of poliovirus protein 2C.
    Virology. 1994 Feb 15;199(1):188-99 PMID: 8116242
  21. Membrane rearrangement and vesicle induction by recombinant poliovirus 2C and 2BC in human cells.
    Virology. 1994 Jul;202(1):129-45 PMID: 8009827
  22. Amino terminal regions of poliovirus 2C protein mediate membrane binding.
    Virology. 1995 Apr 20;208(2):540-53 PMID: 7747426
  23. Human enteric Caliciviridae: the complete genome sequence and expression of virus-like particles from a genetic group II small round structured virus.
    J Gen Virol. 1995 Sep;76 ( Pt 9):2349-55 PMID: 7561776
  24. 3C-like protease of rabbit hemorrhagic disease virus: identification of cleavage sites in the ORF1 polyprotein and analysis of cleavage specificity.
    J Virol. 1995 Nov;69(11):7159-68 PMID: 7474137
  25. Detection of the ORF3 polypeptide of feline calicivirus in infected cells and evidence for its expression from a single, functionally bicistronic, subgenomic mRNA.
    J Gen Virol. 1996 Jan;77 ( Pt 1):123-7 PMID: 8558120
  26. Polyprotein processing in Southampton virus: identification of 3C-like protease cleavage sites by in vitro mutagenesis.
    J Virol. 1996 Apr;70(4):2605-10 PMID: 8642693
  27. Membrane permeabilization by poliovirus proteins 2B and 2BC.
    J Biol Chem. 1996 Sep 20;271(38):23134-7 PMID: 8798506
  28. Membrane permeability induced by hepatitis A virus proteins 2B and 2BC and proteolytic processing of HAV 2BC.
    Virology. 1998 Dec 5;252(1):218-27 PMID: 9875331
  29. Identification of further proteolytic cleavage sites in the Southampton calicivirus polyprotein by expression of the viral protease in E. coli.
    J Gen Virol. 1999 Feb;80 ( Pt 2):291-6 PMID: 10073687
  30. Complete nucleotide sequence and genomic organization of a primate calicivirus, Pan-1.
    Arch Virol. 1999;144(1):199-208 PMID: 10076521
  31. Mapping of the feline calicivirus proteinase responsible for autocatalytic processing of the nonstructural polyprotein and identification of a stable proteinase-polymerase precursor protein.
    J Virol. 1999 Aug;73(8):6626-33 PMID: 10400760
  32. Nucleotide sequence of UK and Australian isolates of feline calicivirus (FCV) and phylogenetic analysis of FCVs.
    Vet Microbiol. 1999 Jun 30;67(3):175-93 PMID: 10418872
  33. Feline calicivirus protein synthesis investigated by western blotting.
    Arch Virol. 1989;108(1-2):69-79 PMID: 2596975
  34. RNA transcripts derived from a cloned full-length copy of the feline calicivirus genome do not require VpG for infectivity.
    Virology. 1995 Jul 10;210(2):383-90 PMID: 7618275
  35. Genetic map of the calicivirus rabbit hemorrhagic disease virus as deduced from in vitro translation studies.
    J Virol. 1996 Nov;70(11):7974-83 PMID: 8892921
  36. Poliovirus infection and expression of the poliovirus protein 2B provoke the disassembly of the Golgi complex, the organelle target for the antipoliovirus drug Ro-090179.
    J Virol. 1997 Jun;71(6):4679-93 PMID: 9151862
  37. Induction of intracellular membrane rearrangements by HAV proteins 2C and 2BC.
    Virology. 1997 Oct 13;237(1):66-77 PMID: 9344908
  38. Cleavage of the feline calicivirus capsid precursor is mediated by a virus-encoded proteinase.
    J Virol. 1998 Apr;72(4):3051-9 PMID: 9525628
  39. Taxonomy of the caliciviruses.
    J Infect Dis. 2000 May;181 Suppl 2:S322-30 PMID: 10804145
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.76.14.7060-7072.2002
Email
ss216m@nih.gov
Published
2002-07-00
Pages
7060-72
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC136307
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.76.14.7060-7072.2002 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2