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PMID: 12039766 Published · ppublish English Journal Article

Isolation and metabolic characteristics of previously uncultured members of the order aquificales in a subsurface gold mine.

Applied and environmental microbiology ·Vol. 68 ·No. 6 ·2002-06-00 ·Pages 3046-54

Takai K, Hirayama H, Sakihama Y, Inagaki F, Yamato Y, Horikoshi K

Abstract

Culture-dependent and -independent techniques were combined to characterize the physiological properties and the ecological impacts of culture-resistant phylotypes of thermophiles within the order Aquificales from a subsurface hot aquifer of a Japanese gold mine. Thermophilic bacteria phylogenetically associated with previously uncultured phylotypes of Aquificales were successfully isolated. 16S ribosomal DNA clone analysis of the entire microbial DNA assemblage and fluorescence in situ whole-cell hybridization analysis indicated that the isolates dominated the microbial population in the subsurface aquifer. The isolates were facultatively anaerobic, hydrogen- or sulfur/thiosulfate-oxidizing, thermophilic chemolithoautotrophs utilizing molecular oxygen, nitrate, ferric iron, arsenate, selenate, and selenite as electron acceptors. Their versatile energy-generating systems may reflect the geochemical conditions of their habitat in the geothermally active subsurface gold mine.

MeSH Terms
Bacteria/isolation & purification,metabolism Colony Count, Microbial DNA, Ribosomal/analysis Gold In Situ Hybridization, Fluorescence Mining Soil Microbiology
Chemicals
DNA, Ribosomal Gold
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Takai Ken
Sunground Animalcule Retrieval (SUGAR) Project, Frontier Research System for Extremophiles, Japan Marine Science & Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. kent@jamstec.go.jp
Hirayama Hisako
Sakihama Yuri
Inagaki Fumio
Yamato Yu
Horikoshi Koki
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.68.6.3046-3054.2002
Email
kent@jamstec.go.jp
Published
2002-06-00
Pages
3046-54
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC123937
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.68.6.3046-3054.2002 PMC Full Text View journal details
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