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PMID: 10584021 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular phylogenetic analysis of archaeal intron-containing genes coding for rRNA obtained from a deep-subsurface geothermal water pool.

Applied and environmental microbiology ·Vol. 65 ·No. 12 ·1999-12-00 ·Pages 5586-9

Takai K, Horikoshi K

Abstract

Molecular phylogenetic analysis of a naturally occurring microbial community in a deep-subsurface geothermal environment indicated that the phylogenetic diversity of the microbial population in the environment was extremely limited and that only hyperthermophilic archaeal members closely related to Pyrobaculum were present. All archaeal ribosomal DNA sequences contained intron-like sequences, some of which had open reading frames with repeated homing-endonuclease motifs. The sequence similarity analysis and the phylogenetic analysis of these homing endonucleases suggested the possible phylogenetic relationship among archaeal rRNA-encoded homing endonucleases.

MeSH Terms

Archaea/classification,genetics,isolation & purification DNA, Archaeal/genetics DNA, Ribosomal/genetics Fresh Water/microbiology Hot Temperature Introns Molecular Sequence Data Open Reading Frames Phylogeny RNA, Archaeal/genetics RNA, Ribosomal/genetics

Chemicals

DNA, Archaeal DNA, Ribosomal RNA, Archaeal RNA, Ribosomal

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Takai K

Deep-sea Microorganisms Research Group, Japan Marine Science & Technology Center, Yokosuka 237-0061, Japan. Ken.Takai@pnl.gov

Horikoshi K

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/AEM.65.12.5586-5589.1999

ken.takai@pnl.gov

Published

1999-12-00

Pages

5586-9

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC91761

Subset

Databases

GENBANK

AB027539, AB027540

External Links

PubMed source DOI: 10.1128/AEM.65.12.5586-5589.1999 PMC Full Text View journal details

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Molecular phylogenetic analysis of archaeal intron-containing genes coding for rRNA obtained from a deep-subsurface geothermal water pool.

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