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PMID: 12034840 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ADA3-containing complexes associate with estrogen receptor alpha.

Nucleic acids research ·Vol. 30 ·No. 11 ·2002-06-01 ·Pages 2508-14

Benecke A, Gaudon C, Garnier JM, vom Baur E, Chambon P, Losson R

Abstract

Transcriptional repression and activation by nuclear receptors (NRs) are brought about by coregulator complexes. These complexes modify the chromatin environment of target genes and affect the activity of the basal transcription machinery. We have previously implicated the yeast ADA3 protein in transcriptional activation by estrogen and retinoid X receptors in yeast and mammalian cells. Here we report the cloning of the mouse homolog of ADA3 and its characterization with respect to the estrogen receptor alpha (ERalpha) function. Mouse mADA3 is 23% identical and 47% similar to yeast yADA3, and mADA3 in contrast to yADA3 does not interact with NRs directly even though it contains two LxxLL NR boxes. However, the ADA3-containing TBP-free-TAF-containing complex (TFTC) can interact with ERalpha in a ligand-independent manner, indicating that other subunits of the complex are sufficient to mediate interaction with NRs.

MeSH Terms
Animals COS Cells Cell Line Estrogen Receptor alpha Expressed Sequence Tags Fungal Proteins/chemistry,genetics Humans Macromolecular Substances Mice Molecular Sequence Data Precipitin Tests Protein Binding Receptors, Estrogen/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology Transcription Factors/chemistry,genetics,metabolism Two-Hybrid System Techniques
Chemicals
Estrogen Receptor alpha Fungal Proteins Macromolecular Substances NGG1 protein, S cerevisiae Receptors, Estrogen Saccharomyces cerevisiae Proteins Tada3 protein, mouse Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Benecke Arndt
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Collège de France, BP163, 67404 Illkirch Cedex, France.
Gaudon Claudine
Garnier Jean-Marie
vom Baur Elmar
Chambon Pierre
Losson Régine
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/30.11.2508
Published
2002-06-01
Pages
2508-14
Language
English
Region
England
NLM ID
0411011
PMCID
PMC117179
Subset
IM
Databases
GENBANK
AF383154
External Links
PubMed source DOI: 10.1093/nar/30.11.2508 PMC Full Text View journal details
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