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PMID: 12024050 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The G(1) cyclin Cln3 promotes cell cycle entry via the transcription factor Swi6.

Molecular and cellular biology ·Vol. 22 ·No. 12 ·2002-06-00 ·Pages 4402-18

Wijnen H, Landman A, Futcher B

Abstract

In Saccharomyces cerevisiae (budding yeast), commitment to cell division in late G(1) is promoted by the G(1) cyclin Cln3 and its associated cyclin-dependent kinase, Cdc28. We show here that all known aspects of the function of Cln3 in G(1) phase, including control of cell size, pheromone sensitivity, cell cycle progress, and transcription, require the protein Swi6. Swi6 is a component of two related transcription factors, SBF and MBF, which are known to regulate many genes at the G(1)-S transition. The Cln3-Cdc28 complex somehow activates SBF and MBF, but there was no evidence for direct phosphorylation of SBF/MBF by Cln3-Cdc28 or for a stable complex between SBF/MBF and Cln3-Cdc28. The activation also does not depend on the ability of Cln3 to activate transcription when artificially recruited directly to a promoter. The amino terminus and the leucine zipper of Swi6 are important for the ability of Swi6 to respond to Cln3 but are not essential for the basal transcriptional activity of Swi6. Cln3-Cdc28 may activate SBF and MBF indirectly, perhaps by phosphorylating some intermediary protein.

MeSH Terms
CDC28 Protein Kinase, S cerevisiae/metabolism Cell Cycle/genetics Cyclins/genetics,metabolism DNA-Binding Proteins Fungal Proteins/genetics,metabolism G1 Phase/genetics Mutation Pheromones/metabolism Phosphorylation Saccharomyces cerevisiae Proteins/genetics,metabolism Transcription Factors/genetics,metabolism Transcription, Genetic Yeasts/genetics,metabolism
Chemicals
CLN3 protein, S cerevisiae Cyclins DNA-Binding Proteins Fungal Proteins MBP1 protein, S cerevisiae Pheromones SWI4 protein, S cerevisiae SWI6 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors CDC28 Protein Kinase, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wijnen Herman
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Landman Allison
Futcher Bruce
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.22.12.4402-4418.2002
Published
2002-06-00
Pages
4402-18
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC133883
Subset
IM
Grants
NIGMS NIH HHS · R01 GM039978 · United States
NIGMS NIH HHS · R01 GM39978 · United States
External Links
PubMed source DOI: 10.1128/MCB.22.12.4402-4418.2002 PMC Full Text View journal details
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