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PMID: 12000852 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Telomere measurement by quantitative PCR.

Nucleic acids research ·Vol. 30 ·No. 10 ·2002-05-15 ·Pages e47

Cawthon RM

Abstract

It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. This assay will facilitate investigations of the biology of telomeres and the roles they play in the molecular pathophysiology of diseases and aging.

MeSH Terms
Adolescent Adult Aged Aged, 80 and over Base Sequence Child Child, Preschool DNA/genetics DNA Primers/genetics Electrophoresis, Agar Gel Female Humans Male Middle Aged Molecular Sequence Data Polymerase Chain Reaction/methods Reproducibility of Results Telomere/genetics
Chemicals
DNA Primers DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Cawthon Richard M
Department of Human Genetics, University of Utah, 15 N 2030 E, Room 2100, Salt Lake City, UT 84112, USA. rcawthon@genetics.utah.edu
References (12)
12 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/30.10.e47
Email
rcawthon@genetics.utah.edu
Published
2002-05-15
Pages
e47
Language
English
Region
England
NLM ID
0411011
PMCID
PMC115301
Subset
IM
Grants
Intramural NIH HHS · Z01 AG000767 · United States
NIA NIH HHS · AG13478 · United States
NIA NIH HHS · K01 AG00767 · United States
NCI NIH HHS · R29CA69421 · United States
External Links
PubMed source DOI: 10.1093/nar/30.10.e47 PMC Full Text View journal details
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