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PMID: 11983867 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Dynamic assembly of MinD on phospholipid vesicles regulated by ATP and MinE.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 10 ·2002-05-14 ·Pages 6761-6

Hu Z, Gogol EP, Lutkenhaus J

Abstract

Selection of the division site in Escherichia coli is regulated by the min system and requires the rapid oscillation of MinD between the two halves of the cell under the control of MinE. In this study we have further investigated the molecular basis for this oscillation by examining the interaction of MinD with phospholipid vesicles. We found that MinD bound to phospholipid vesicles in the presence of ATP and, upon binding, assembled into a well-ordered helical array that deformed the vesicles into tubes. Stimulation of the MinD ATPase by addition of MinE led to disassembly of the tubes and the release of MinD from the vesicles. It is proposed that this MinE-regulated dynamic assembly of MinD underlies MinD oscillation.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Adenosine Triphosphate/metabolism Bacterial Proteins/metabolism Cell Cycle Proteins Escherichia coli/genetics,metabolism,ultrastructure Escherichia coli Proteins Phospholipids/metabolism Recombinant Fusion Proteins/genetics,metabolism
Chemicals
Bacterial Proteins Cell Cycle Proteins Escherichia coli Proteins MinE protein, E coli Phospholipids Recombinant Fusion Proteins Adenosine Triphosphate Adenosine Triphosphatases MinD protein, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hu Zonglin
Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, USA.
Gogol Edward P
Lutkenhaus Joe
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.102059099
Published
2002-05-14
Epub
2002-00-30
Pages
6761-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC124476
Subset
IM
Grants
NIGMS NIH HHS · R01 GM029764 · United States
NIGMS NIH HHS · R37 GM029764 · United States
NIGMS NIH HHS · GM 29764 · United States
External Links
PubMed source DOI: 10.1073/pnas.102059099 PMC Full Text View journal details
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