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PMID: 11967383 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A model of the replication fork blocking protein Fob1p based on the catalytic core domain of retroviral integrases.

Protein science : a publication of the Protein Society ·Vol. 11 ·No. 5 ·2002-05-00 ·Pages 1274-7

Dlakić M

Abstract

The replication fork blocks are common in both prokaryotes and eukaryotes. In most cases, these blocks are associated with increased levels of mitotic recombination. One of the best-characterized replication fork blocks in eukaryotes is found in ribosomal DNA (rDNA) repeats of Saccharomyces cerevisiae. It has been shown that the replication fork blocking protein Fob1p regulates the recombination rate and the number of rDNA copies in S. cerevisiae, but the mechanistic aspects of these events are still poorly understood. Sequence profile searches revealed that Fob1p is related to retroviral integrases. Subsequently, the catalytic domain of HIV-1 integrase was used as a template to build a reliable three-dimensional model of Fob1p. Structural insights from this study may be useful in explaining Fob1p-mediated formation of extrachromosomal rDNA circles that accelerate aging in yeast and recombination events that lead to expansion or contraction of rDNA.

MeSH Terms
Amino Acid Sequence Catalytic Domain DNA-Binding Proteins Fungal Proteins/chemistry HIV Integrase/chemistry Models, Molecular Molecular Sequence Data Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Analysis, Protein
Chemicals
DNA-Binding Proteins FOB1 protein, S cerevisiae Fungal Proteins Saccharomyces cerevisiae Proteins HIV Integrase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Dlakić Mensur
Department of Biological Chemistry, The University of Michigan Medical School, Ann Arbor, MI 48109-0606, USA. mensur@umich.edu
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Article Info
Journal
Protein science : a publication of the Protein Society
Abbr.
Protein Sci
ISSN
0961-8368
DOI
10.1110/ps.4470102
Email
mensur@umich.edu
Published
2002-05-00
Pages
1274-7
Language
English
Region
United States
NLM ID
9211750
PMCID
PMC2373559
Subset
IM
External Links
PubMed source DOI: 10.1110/ps.4470102 PMC Full Text View journal details
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