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PMID: 11932436 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cyclophilin a plays distinct roles in human immunodeficiency virus type 1 entry and postentry events, as revealed by spinoculation.

Journal of virology ·Vol. 76 ·No. 9 ·2002-05-00 ·Pages 4671-7

Saphire AC, Bobardt MD, Gallay PA

Abstract

Cyclophilin A (CypA) is necessary for effective human immunodeficiency virus type 1 (HIV-1) replication. However, the functions of CypA and the precise steps at which CypA acts in the HIV-1 life cycle remain to be determined. By using a methodology that bypasses the need for attachment factors-spinoculation-we present evidence that CypA participates in both entry and postentry events.

MeSH Terms
Centrifugation/methods Cyclophilin A/metabolism Enzyme-Linked Immunosorbent Assay HIV-1/pathogenicity,physiology HeLa Cells Humans Polysaccharide-Lyases/metabolism
Chemicals
Polysaccharide-Lyases heparitinsulfate lyase Cyclophilin A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Saphire Andrew C S
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA.
Bobardt Michael D
Gallay Philippe A
References (29)
29 references, click to expand
  1. CD147 facilitates HIV-1 infection by interacting with virus-associated cyclophilin A.
    Proc Natl Acad Sci U S A. 2001 May 22;98(11):6360-5 PMID: 11353871
  2. Syndecans serve as attachment receptors for human immunodeficiency virus type 1 on macrophages.
    J Virol. 2001 Oct;75(19):9187-200 PMID: 11533182
  3. Cyclophilin A regulates HIV-1 infectivity, as demonstrated by gene targeting in human T cells.
    EMBO J. 2001 Mar 15;20(6):1300-9 PMID: 11250896
  4. Human immunodeficiency virus type 1 spinoculation enhances infection through virus binding.
    J Virol. 2000 Nov;74(21):10074-80 PMID: 11024136
  5. Human immunodeficiency virus type 1 entry into macrophages mediated by macropinocytosis.
    J Virol. 2001 Nov;75(22):11166-77 PMID: 11602756
  6. trans-Complementation rescue of cyclophilin A-deficient viruses reveals that the requirement for cyclophilin A in human immunodeficiency virus type 1 replication is independent of its isomerase activity.
    J Virol. 2002 Mar;76(5):2255-62 PMID: 11836403
  7. Cyclophilin: a specific cytosolic binding protein for cyclosporin A.
    Science. 1984 Nov 2;226(4674):544-7 PMID: 6238408
  8. Protein folding in the cell.
    Nature. 1992 Jan 2;355(6355):33-45 PMID: 1731198
  9. Human immunodeficiency virus type 1 Gag protein binds to cyclophilins A and B.
    Cell. 1993 Jun 18;73(6):1067-78 PMID: 8513493
  10. Vif is crucial for human immunodeficiency virus type 1 proviral DNA synthesis in infected cells.
    J Virol. 1993 Aug;67(8):4945-55 PMID: 8331734
  11. The nuclear localization signal of the matrix protein of human immunodeficiency virus type 1 allows the establishment of infection in macrophages and quiescent T lymphocytes.
    Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):6992-6 PMID: 8041734
  12. Crystal structures of cyclophilin A complexed with cyclosporin A and N-methyl-4-[(E)-2-butenyl]-4,4-dimethylthreonine cyclosporin A.
    Structure. 1994 Jan 15;2(1):33-44 PMID: 8075981
  13. Specific incorporation of cyclophilin A into HIV-1 virions.
    Nature. 1994 Nov 24;372(6504):359-62 PMID: 7969494
  14. Functional association of cyclophilin A with HIV-1 virions.
    Nature. 1994 Nov 24;372(6504):363-5 PMID: 7969495
  15. Mode of action of SDZ NIM 811, a nonimmunosuppressive cyclosporin A analog with activity against human immunodeficiency virus (HIV) type 1: interference with HIV protein-cyclophilin A interactions.
    J Virol. 1995 Apr;69(4):2451-61 PMID: 7884893
  16. Cyclophilin A is required for an early step in the life cycle of human immunodeficiency virus type 1 before the initiation of reverse transcription.
    J Virol. 1996 Jun;70(6):3551-60 PMID: 8648689
  17. Binding of the human immunodeficiency virus type 1 Gag polyprotein to cyclophilin A is mediated by the central region of capsid and requires Gag dimerization.
    J Virol. 1996 Jul;70(7):4299-310 PMID: 8676452
  18. Inhibition of HIV-1 replication by cyclosporine A or related compounds correlates with the ability to disrupt the Gag-cyclophilin A interaction.
    Virology. 1996 Aug 1;222(1):279-82 PMID: 8806510
  19. Absconding with the chaperone: essential cyclophilin-Gag interaction in HIV-1 virions.
    Cell. 1996 Dec 27;87(7):1157-9 PMID: 8980222
  20. Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsid.
    Cell. 1996 Dec 27;87(7):1285-94 PMID: 8980234
  21. The hydrophobic pocket of cyclophilin is the binding site for the human immunodeficiency virus type 1 Gag polyprotein.
    J Virol. 1997 Mar;71(3):2107-13 PMID: 9032343
  22. The non-immunosuppressive cyclosporin A analogue SDZ NIM 811 inhibits cyclophilin A incorporation into virions and virus replication in human immunodeficiency virus type 1-infected primary and growth-arrested T cells.
    J Gen Virol. 1997 Apr;78 ( Pt 4):825-35 PMID: 9129655
  23. Molecular recognition in the HIV-1 capsid/cyclophilin A complex.
    J Mol Biol. 1997 Jun 27;269(5):780-95 PMID: 9223641
  24. Active-site residues of cyclophilin A are crucial for its incorporation into human immunodeficiency virus type 1 virions.
    J Virol. 1997 Sep;71(9):7110-3 PMID: 9261445
  25. HIV-1 infection of nondividing cells through the recognition of integrase by the importin/karyopherin pathway.
    Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9825-30 PMID: 9275210
  26. Functional RT and IN incorporated into HIV-1 particles independently of the Gag/Pol precursor protein.
    EMBO J. 1997 Aug 15;16(16):5113-22 PMID: 9305652
  27. Role of cyclophilin A in the uptake of HIV-1 by macrophages and T lymphocytes.
    Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1758-63 PMID: 9465090
  28. Human immunodeficiency virus type 1 attachment to HeLa CD4 cells is CD4 independent and gp120 dependent and requires cell surface heparans.
    J Virol. 1998 May;72(5):3623-34 PMID: 9557643
  29. Host cyclophilin A mediates HIV-1 attachment to target cells via heparans.
    EMBO J. 1999 Dec 1;18(23):6771-85 PMID: 10581250
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.76.9.4671-4677.2002
Published
2002-05-00
Pages
4671-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC155090
Subset
IM
Grants
NIAID NIH HHS · R01 AI046958 · United States
NIAID NIH HHS · R21 AI046958 · United States
NIAID NIH HHS · AI46958 · United States
External Links
PubMed source DOI: 10.1128/jvi.76.9.4671-4677.2002 PMC Full Text View journal details
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