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PMID: 11353871 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

CD147 facilitates HIV-1 infection by interacting with virus-associated cyclophilin A.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 11 ·2001-05-22 ·Pages 6360-5

Pushkarsky T, Zybarth G, Dubrovsky L, Yurchenko V, Tang H, Guo H, Toole B, Sherry B, Bukrinsky M

Abstract

Cyclophilin A (CyPA) is specifically incorporated into the virions of HIV-1 and has been shown to enhance significantly an early step of cellular HIV-1 infection. Our preliminary studies implicated CD147 as a receptor for extracellular CyPA. Here, we demonstrate a role for CyPA-CD147 interaction during the early steps of HIV-1 infection. Expression of human CD147 increased infection by HIV-1 under one-cycle conditions. However, susceptibility to infection by viruses lacking CyPA (simian immunodeficiency virus or HIV-1 produced in the presence of cyclosporin A) was unaffected by CD147. Virus-associated CyPA coimmunoprecipitated with CD147 from infected cells. Antibody to CD147 inhibited HIV-1 entry as evidenced by the delay in translocation of the HIV-1 core proteins from the membrane and inhibition of viral reverse transcription. Viruses whose replication did not require CyPA (SIV or mutant HIV-1) were resistant to the inhibitory effect of anti-CD147 antibody. These results suggest that HIV-1 entry depends on an interaction between virus-associated CyPA and CD147 on a target cell.

MeSH Terms
Animals Antibodies, Monoclonal/metabolism Antigens, CD Antigens, Neoplasm Antigens, Surface Avian Proteins Basigin Blood Proteins CHO Cells Cricetinae Cyclophilin A/metabolism HIV-1/metabolism,physiology Humans Membrane Glycoproteins/genetics,metabolism
Chemicals
Antibodies, Monoclonal Antigens, CD Antigens, Neoplasm Antigens, Surface Avian Proteins BSG protein, human Blood Proteins Bsg protein, Gallus gallus Bsg protein, rat Membrane Glycoproteins Basigin Cyclophilin A
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Pushkarsky T
The Picower Institute for Medical Research, 350 Community Drive, Manhasset, NY 11030, USA.
Zybarth G
Dubrovsky L
Yurchenko V
Tang H
Guo H
Toole B
Sherry B
Bukrinsky M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.111583198
Published
2001-05-22
Epub
2001-00-15
Pages
6360-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC33473
Subset
IM
Grants
NIAID NIH HHS · R01 AI 29110 · United States
NIAID NIH HHS · R01- AI 38245 · United States
External Links
PubMed source DOI: 10.1073/pnas.111583198 PMC Full Text View journal details
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