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PMID: 11927555 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The fission yeast NIMA kinase Fin1p is required for spindle function and nuclear envelope integrity.

The EMBO journal ·Vol. 21 ·No. 7 ·2002-04-02 ·Pages 1713-22

Krien MJ, West RR, John UP, Koniaras K, McIntosh JR, O'Connell MJ

Abstract

NIMA kinases appear to be the least functionally conserved mitotic regulators, being implicated in chromosome condensation in fungi and in spindle function in metazoans. We demonstrate here that the fission yeast NIMA homologue, Fin1p, can induce profound chromosome condensation in the absence of the condensin and topoisomerase II, indicating that Fin1p-induced condensation differs from mitotic condensation. Fin1p expression is transcriptionally and post-translationally cell cycle-regulated, with Fin1p kinase activity maximal from the metaphase-anaphase transition to G(1). Fin1p is localized to the spindle pole body and fin1Delta cells are hypersensitive to anti-microtubule drugs, synthetically lethal with a number of spindle mutants and require the spindle checkpoint for viability. Moreover, fin1Delta cells show unusual and extensive elaborations of the nuclear envelope. These data support a role for Fin1p in spindle function and nuclear envelope transactions at or after the metaphase-anaphase transition that may be generally applicable to other NIMA-family members.

MeSH Terms
Cell Cycle Cell Cycle Proteins Chromosomes, Fungal/physiology NIMA-Related Kinase 1 Nuclear Envelope/physiology Protein Serine-Threonine Kinases/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Schizosaccharomyces/enzymology,genetics,metabolism,physiology Spindle Apparatus/physiology
Chemicals
Cell Cycle Proteins Recombinant Fusion Proteins NIMA-Related Kinase 1 Protein Serine-Threonine Kinases fin1 protein, S pombe
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Krien Michael J E
Trescowthick Research Laboratories, Peter MacCallum Cancer Institute, Locked Bag 1, A'Beckett Street, Melbourne, Victoria 8006, Australia.
West Robert R
John Ulrik P
Koniaras Kalli
McIntosh J Richard
O'Connell Matthew J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/21.7.1713
Published
2002-04-02
Pages
1713-22
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125934
Subset
IM
Grants
NIGMS NIH HHS · R01 GM033787 · United States
NIGMS NIH HHS · GM-33787 · United States
External Links
PubMed source DOI: 10.1093/emboj/21.7.1713 PMC Full Text View journal details
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