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PMID: 11904391 Published · ppublish English Journal Article

Gramicidin A channels switch between stretch activation and stretch inactivation depending on bilayer thickness.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 99 ·No. 7 ·2002-04-02 ·Pages 4308-12

Martinac B, Hamill OP

Abstract

The patch clamp-liposome technique was used to examine the stretch sensitivity of a model membrane ion channel, gramicidin A, in membrane patches of different bilayer thickness. We found that small changes in phospholipid acyl chain length (i.e., PC-20 to PC-18) can switch gramicidin A from a stretch-activated to a stretch-inactivated channel. The demonstration that subnanometer changes in bilayer thickness can reverse the response polarity of a model channel has implications for other signaling proteins that may experience local changes in bilayer thickness as a consequence of dynamic targeting to lipid microdomains, electrocompression, or chemical modification of the bilayer.

MeSH Terms
Gramicidin/chemistry,metabolism Ion Channels/chemistry Lipid Bilayers/chemistry Patch-Clamp Techniques
Chemicals
Ion Channels Lipid Bilayers Gramicidin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Martinac Boris
Department of Pharmacology, University of Western Australia, Crawley, West Australia 6009.
Hamill Owen P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.072632899
Published
2002-04-02
Epub
2002-00-19
Pages
4308-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC123644
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.072632899 PMC Full Text View journal details
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