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PMID: 11903063 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells.

The Biochemical journal ·Vol. 363 ·No. Pt 1 ·2002-04-01 ·Pages 195-200

Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH

Abstract

Dysregulation of the human transforming acidic coiled-coil (TACC) proteins is thought to be important in the evolution of breast cancer and multiple myeloma. However, the exact role of these proteins in the oncogenic process is currently unknown. Using the full-length TACC1 protein as bait to screen a human mammary epithelial cDNA library, we have identified two genes that are also amplified and overexpressed in tumours derived from different cellular origins. TACC1 interacts with the C-terminus of both the microtubule-associated colonic and hepatic tumour overexpressed (ch-TOG) protein, and the oncogenic transcription factor glioma amplified sequence 41/NuMA binding protein 1 (GAS41/NuBI1; where NuMA stands for nuclear mitotic apparatus protein 1). This suggests that the TACC proteins can form multiple complexes, dysregulation of which may be an important step during tumorigenesis.

MeSH Terms
Amino Acid Sequence Blotting, Western Breast Neoplasms/metabolism Cell Line Cell Nucleus/metabolism DNA, Complementary/metabolism Fetal Proteins Fluorescent Antibody Technique, Indirect Gene Library Green Fluorescent Proteins Humans Luminescent Proteins/metabolism Microtubule-Associated Proteins/chemistry,metabolism Microtubules/metabolism Molecular Sequence Data Nuclear Proteins Precipitin Tests Protein Binding Protein Structure, Tertiary Sequence Homology, Amino Acid Signal Transduction Transcription Factors/metabolism Transcription, Genetic Tumor Cells, Cultured Two-Hybrid System Techniques
Chemicals
CKAP5 protein, human DNA, Complementary Fetal Proteins Luminescent Proteins Microtubule-Associated Proteins Nuclear Proteins TACC1 protein, human Transcription Factors YEATS4 protein, human Green Fluorescent Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lauffart Brenda
Department of Cancer Genetics, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA. Brenda.Laufert@roswellpark.org
Howell Scott J
Tasch Jason E
Cowell John K
Still Ivan H
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/0264-6021:3630195
Email
brenda.laufert@roswellpark.org
Published
2002-04-01
Pages
195-200
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1222467
Subset
IM
Grants
NCI NIH HHS · CA16056 · United States
External Links
PubMed source DOI: 10.1042/0264-6021:3630195 PMC Full Text View journal details
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