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PMID: 11895963 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Involvement of PhoP-PhoS homologs in Enterococcus faecalis virulence.

Infection and immunity ·Vol. 70 ·No. 4 ·2002-04-00 ·Pages 1991-6

Teng F, Wang L, Singh KV, Murray BE, Weinstock GM

Abstract

Eleven PhoP-PhoS homolog pairs were identified by searching the Enterococcus faecalis V583 genome sequence database at The Institute for Genomic Research with the Bacillus subtilis PhoP-PhoS sequences. Each pair appears to be a potential two-component system composed of a response regulator and a sensor kinase. Seven of the homologs were disrupted in E. faecalis strain OG1RF. TX10293, a mutant disrupted in one of these genes (etaR, the first gene of the gene pair designated etaRS), showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. The predicted EtaR protein sequence showed greatest similarity to LisR of Listeria monocytogenes (77%) and CsrR of Streptococcus pyogenes (70%); EtaS is 53% similar to LisK and 54% similar to CsrS. When grown in vitro, the TX10293 mutant was more sensitive to low pH (pH 3.4) and more resistant to high temperature (55 degrees C) than wild-type OG1RF. In conclusion, many potential two-component systems are identified for E. faecalis, one of which, EtaRS, was shown to be involved in stress response and virulence.

MeSH Terms

Amino Acid Sequence Animals Bacterial Proteins/genetics,physiology Enterococcus faecalis/genetics,growth & development,pathogenicity Genes, Bacterial Genes, Regulator Hydrogen-Ion Concentration Mice Molecular Sequence Data Protein Kinases/chemistry Temperature Virulence/genetics

Chemicals

Bacterial Proteins PhoP protein, Bacteria Protein Kinases CsrS protein, bacteria

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Teng Fang

Department of Microbiology and Molecular Genetics, University of Texas Houston Medical School, Houston, Texas 77030, USA.

Wang Ling

Singh Kavindra V

Murray Barbara E

Weinstock George M

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19 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/IAI.70.4.1991-1996.2002

Published

2002-04-00

Pages

1991-6

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC127847

Subset

Grants

NIAID NIH HHS · R01 AI047923 · United States

NIAID NIH HHS · R37 AI047923 · United States

NIAID NIH HHS · AI47923 · United States

External Links

PubMed source DOI: 10.1128/IAI.70.4.1991-1996.2002 PMC Full Text View journal details

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Involvement of PhoP-PhoS homologs in Enterococcus faecalis virulence.

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