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PMID: 10639451 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of a gene cluster of Enterococcus faecalis involved in polysaccharide biosynthesis.

Infection and immunity ·Vol. 68 ·No. 2 ·2000-02-00 ·Pages 815-23

Xu Y, Singh KV, Qin X, Murray BE, Weinstock GM

Abstract

Previously, we described a gene cluster of Enterococcus faecalis OG1RF that produced an antigenic polysaccharide when cloned in Escherichia coli. The polysaccharide antigen was not detectable in E. faecalis strains, however. Here, we show by reverse transcriptase-PCR that the 16 genes in this region are transcribed in OG1RF. Gene disruption of orfde4, encoding a putative glycosyl transferase, and orfde6, a putative dTDP-rhamnose biosynthesis gene, generated two OG1RF mutants. The mutants showed delayed killing and a higher 50% lethal dose in a mouse peritonitis model. In addition, two mucoid E. faecalis isolates from patients with chronic urinary tract infections were found to produce the polysaccharide antigen.

MeSH Terms
Animals Enterococcus faecalis/genetics,growth & development,immunology Genes, Bacterial Humans Mice Multigene Family Mutation Open Reading Frames Peritonitis/etiology Polysaccharides, Bacterial/biosynthesis,immunology RNA, Messenger/analysis Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic
Chemicals
Polysaccharides, Bacterial RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Xu Y
Department of Microbiology and Molecular Genetics, University of Texas Medical School, Houston, Texas 77030, USA.
Singh K V
Qin X
Murray B E
Weinstock G M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.68.2.815-823.2000
Published
2000-02-00
Pages
815-23
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC97210
Subset
IM
Grants
NIAID NIH HHS · AI33516 · United States
External Links
PubMed source DOI: 10.1128/IAI.68.2.815-823.2000 PMC Full Text View journal details
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