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PMID: 11872498 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and genotyping of Helicobacter pylori from untreated municipal wastewater.

Applied and environmental microbiology ·Vol. 68 ·No. 3 ·2002-03-00 ·Pages 1436-9

Lu Y, Redlinger TE, Avitia R, Galindo A, Goodman K

Abstract

For this study, we isolated Helicobacter pylori from wastewater by a series of steps beginning with immunomagnetic separation and cell culture. After Gram staining and three standard microbial tests, the 16S rRNA sequences of a total of 23 out of 37 putative H. pylori isolates were verified by PCR. Eleven H. pylori isolates were genotyped and fell into four vacA classes: those with the vacA allelic variants s1a and m1, s1b and m1, s2 and m2, or s2 and m1. Most H. pylori isolates were of the vacA s1a/m1 type, which has been shown to be associated with advanced diseases based on genotyping of H. pylori from gastric cancer patients. These results demonstrated that H. pylori survives in water and may be a potential source of H. pylori transmission, especially where water is not adequately treated.

MeSH Terms
Bacterial Proteins/genetics Culture Media DNA, Ribosomal/analysis Genotype Helicobacter pylori/classification,genetics,isolation & purification Immunomagnetic Separation Molecular Sequence Data Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Sewage/microbiology
Chemicals
Bacterial Proteins Culture Media DNA, Ribosomal RNA, Ribosomal, 16S Sewage VacA protein, Helicobacter pylori
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lu Yingzhi
Department of Biological Sciences, University of Texas, El Paso, TX 79968, USA.
Redlinger Thomas E
Avitia Raquel
Galindo Adriana
Goodman Karen
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.68.3.1436-1439.2002
Published
2002-03-00
Pages
1436-9
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC123781
Subset
IM
Grants
NCRR NIH HHS · G12 RR008124 · United States
NCRR NIH HHS · G12 RR 08124 · United States
External Links
PubMed source DOI: 10.1128/AEM.68.3.1436-1439.2002 PMC Full Text View journal details
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