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PMID: 11836431 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Translation of polioviral mRNA is inhibited by cleavage of polypyrimidine tract-binding proteins executed by polioviral 3C(pro).

Journal of virology ·Vol. 76 ·No. 5 ·2002-03-00 ·Pages 2529-42

Back SH, Kim YK, Kim WJ, Cho S, Oh HR, Kim JE, Jang SK

Abstract

The translation of polioviral mRNA occurs through an internal ribosomal entry site (IRES). Several RNA-binding proteins, such as polypyrimidine tract-binding protein (PTB) and poly(rC)-binding protein (PCBP), are required for the poliovirus IRES-dependent translation. Here we report that a poliovirus protein, 3C(pro) (and/or 3CD(pro)), cleaves PTB isoforms (PTB1, PTB2, and PTB4). Three 3C(pro) target sites (one major target site and two minor target sites) exist in PTBs. PTB fragments generated by poliovirus infection are redistributed to the cytoplasm from the nucleus, where most of the intact PTBs are localized. Moreover, these PTB fragments inhibit polioviral IRES-dependent translation in a cell-based assay system. We speculate that the proteolytic cleavage of PTBs may contribute to the molecular switching from translation to replication of polioviral RNA.

MeSH Terms
3C Viral Proteases Amino Acid Sequence Cell Line Cysteine Endopeptidases/genetics,metabolism HeLa Cells Humans Molecular Sequence Data Mutagenesis, Site-Directed Poliovirus/genetics,metabolism Polypyrimidine Tract-Binding Protein Protein Biosynthesis Protein Isoforms/metabolism RNA, Messenger/genetics,metabolism RNA, Viral/genetics,metabolism RNA-Binding Proteins/metabolism Ribonucleoproteins/metabolism Ribosomes/metabolism Subcellular Fractions Viral Proteins/genetics,metabolism
Chemicals
Protein Isoforms RNA, Messenger RNA, Viral RNA-Binding Proteins Ribonucleoproteins Viral Proteins Polypyrimidine Tract-Binding Protein Cysteine Endopeptidases 3C Viral Proteases 3C proteases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Back Sung Hoon
NRL, Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Kyungbuk 790-784, Korea.
Kim Yoon Ki
Kim Woo Jae
Cho Sungchan
Oh Hoe Rang
Kim Jung-Eun
Jang Sung Key
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.76.5.2529-2542.2002
Published
2002-03-00
Pages
2529-42
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC135932
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.76.5.2529-2542.2002 PMC Full Text View journal details
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