Home LiteratureArticle Details
PMID: 11823230 Published · ppublish English Evaluation Study Journal Article Research Support, Non-U.S. Gov't

Highly different levels of natural transformation are associated with genomic subgroups within a local population of Pseudomonas stutzeri from soil.

Applied and environmental microbiology ·Vol. 68 ·No. 2 ·2002-02-00 ·Pages 865-73

Sikorski J, Teschner N, Wackernagel W

Abstract

A highly sensitive and specific PCR-based method of monitoring 16S rRNA genes of Pseudomonas stutzeri was developed for searching P. stutzeri DNA in environmental samples. This monitoring was combined with a reliable and sensitive method for isolating P. stutzeri colony formers from soil and sediment, depending on their utilization of ethylene glycol, starch, and maltose. With these techniques, P. stutzeri populations (n = 2 to 170) were obtained from five of six sites giving positive PCR signals (including three marine sediment and two soil samples). The phylogenetic positions of isolates from the five sites, based on their 16S ribosomal DNA sequences, indicated that the environmental isolates were affiliated with different genomovars of P. stutzeri. Using the broad-host-range plasmid pNS1 with kanamycin and gentamicin resistance determinants as the transforming DNA, naturally transformable strains were identified among the isolates from all sites. For one population from soil, the genetic relationship of the 120 members was determined by randomly amplified polymorphic DNA-PCR with three PCR primers. Among the population members which are taxonomically closely related as determined by 16S sequence comparisons of group representatives, a rather high genetic diversity and a characteristic clustering into subgroups were found. Remarkably, within the population, nontransformability and different levels of transformability (a frequency between about 10(-9) and 10(-4) per cell) were often associated with distinct genetic subgroups. It is concluded that transformability is widespread among environmental P. stutzeri strains and that its specific level is a heritable trait that may vary strongly within a local population.

MeSH Terms
DNA, Ribosomal/analysis Genome, Bacterial Geologic Sediments/microbiology Molecular Sequence Data Phylogeny Plasmids/genetics Polymerase Chain Reaction/methods Pseudomonas/genetics,growth & development,isolation & purification RNA, Ribosomal, 16S/genetics Random Amplified Polymorphic DNA Technique Seawater/microbiology Sensitivity and Specificity Sequence Analysis, DNA Soil Microbiology Transformation, Bacterial/genetics
Chemicals
DNA, Ribosomal RNA, Ribosomal, 16S
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sikorski Johannes
Genetics Section, Department of Biology, University of Oldenburg, D-26111 Oldenburg, Germany.
Teschner Nicole
Wackernagel Wilfried
References (42)
42 references, click to expand
  1. Exchange of chromosomal markers by natural transformation between the soil isolate, Pseudomonas stutzeri JM300, and the marine isolate, Pseudomonas stutzeri strain ZoBell.
    Antonie Van Leeuwenhoek. 1991 Jan;59(1):19-25 PMID: 2059008
  2. Comparative biochemical and genetic analysis of naphthalene degradation among Pseudomonas stutzeri strains.
    Appl Environ Microbiol. 1994 Mar;60(3):966-72 PMID: 8161187
  3. Analysis of genotypic diversity and relationships among Pseudomonas stutzeri strains by PCR-based genomic fingerprinting and multilocus enzyme electrophoresis.
    Syst Appl Microbiol. 1999 Sep;22(3):393-402 PMID: 10553292
  4. TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment.
    Comput Appl Biosci. 1994 Sep;10(5):569-70 PMID: 7828077
  5. 16S rRNA gene sequence analysis relative to genomovars of Pseudomonas stutzeri and proposal of Pseudomonas balearica sp. nov.
    Int J Syst Bacteriol. 1996 Jan;46(1):200-5 PMID: 8573496
  6. Evolution of microbial diversity during prolonged starvation.
    Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):4023-7 PMID: 10097156
  7. A novel peptidoglycan-linked lipoprotein (ComL) that functions in natural transformation competence of Neisseria gonorrhoeae.
    Mol Microbiol. 1996 Mar;19(5):1095-105 PMID: 8830266
  8. Molecular Methods for the Detection and Identification of Pseudomonas stutzeri in Pure Culture and Environmental Samples
    Microb Ecol. 1998 Jan;35(1):22-33 PMID: 9459656
  9. Genome and fatty acid analysis of Pseudomonas stutzeri.
    Int J Syst Bacteriol. 1994 Jan;44(1):54-61 PMID: 8123562
  10. Microbial communities of printing paper machines.
    J Appl Microbiol. 1998 Jun;84(6):1069-84 PMID: 9717292
  11. Genetic analysis of mutagenesis in aging Escherichia coli colonies.
    Mol Gen Genet. 1997 Oct;256(3):277-81 PMID: 9393452
  12. Cell biology and molecular basis of denitrification.
    Microbiol Mol Biol Rev. 1997 Dec;61(4):533-616 PMID: 9409151
  13. Random amplified polymorphic DNA fingerprinting of Campylobacter jejuni and C. coli isolated from human faeces, seawater and poultry products.
    Res Microbiol. 1995 Oct;146(8):685-96 PMID: 8584791
  14. Genes involved in the biogenesis and function of type-4 fimbriae in Pseudomonas aeruginosa.
    Gene. 1997 Jun 11;192(1):89-98 PMID: 9224878
  15. Requirement of novel competence genes pilT and pilU of Pseudomonas stutzeri for natural transformation and suppression of pilT deficiency by a hexahistidine tag on the type IV pilus protein PilAI.
    J Bacteriol. 2001 Aug;183(16):4694-701 PMID: 11466271
  16. 16S ribosomal DNA amplification for phylogenetic study.
    J Bacteriol. 1991 Jan;173(2):697-703 PMID: 1987160
  17. Typing Listeria monocytogenes: a comparison of random amplification of polymorphic DNA with 5 other methods.
    Res Microbiol. 1995 Jan;146(1):35-49 PMID: 7754227
  18. High Frequency of Natural Genetic Transformation of Pseudomonas stutzeri in Soil Extract Supplemented with a Carbon/Energy and Phosphorus Source.
    Appl Environ Microbiol. 1991 Apr;57(4):1246-51 PMID: 16348463
  19. Escherichia-Pseudomonas shuttle vectors derived from pUC18/19.
    Gene. 1991 Jan 2;97(1):109-21 PMID: 1899844
  20. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
    Nucleic Acids Res. 1997 Dec 15;25(24):4876-82 PMID: 9396791
  21. The stationary phase of the bacterial life cycle.
    Annu Rev Microbiol. 1993;47:855-74 PMID: 8257118
  22. Type IV pilus genes pilA and pilC of Pseudomonas stutzeri are required for natural genetic transformation, and pilA can be replaced by corresponding genes from nontransformable species.
    J Bacteriol. 2000 Apr;182(8):2184-90 PMID: 10735861
  23. Mathematical model for studying genetic variation in terms of restriction endonucleases.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269-73 PMID: 291943
  24. Pseudomonas stutzeri nitrite reductase gene abundance in environmental samples measured by real-time PCR.
    Appl Environ Microbiol. 2001 Feb;67(2):760-8 PMID: 11157241
  25. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria.
    Gene. 1988 Oct 15;70(1):191-7 PMID: 2853689
  26. Natural genetic transformation of Pseudomonas stutzeri in a non-sterile soil.
    Microbiology. 1998 Feb;144 ( Pt 2):569-76 PMID: 9493393
  27. Selective isolation of Pseudomonas stutzeri from vertebrate faeces on Rambach agar.
    Zentralbl Bakteriol. 1998 Nov;288(3):343-9 PMID: 9861678
  28. Clonal population structure of Pseudomonas stutzeri, a species with exceptional genetic diversity.
    J Bacteriol. 2001 Jan;183(2):736-44 PMID: 11133969
  29. Pseudomonas stutzeri has two closely related pilA genes (Type IV pilus structural protein) with opposite influences on natural genetic transformation.
    J Bacteriol. 2001 Apr;183(7):2359-66 PMID: 11244078
  30. Transformation of Acinetobacter calco-aceticus (Bacterium anitratum).
    J Bacteriol. 1969 Apr;98(1):281-8 PMID: 5781579
  31. Genetic variability and adaptation to stress.
    EXS. 1997;83:271-90 PMID: 9342853
  32. DNA uptake in bacteria.
    Annu Rev Microbiol. 1999;53:217-44 PMID: 10547691
  33. Occurrence and antibiotic-resistance of Pseudomonas species isolated from drinking water in southern Greece.
    J Chemother. 1994 Apr;6(2):111-6 PMID: 8077985
  34. Use of polymerase chain reaction and electroporation of Escherichia coli to monitor the persistence of extracellular plasmid DNA introduced into natural soils.
    Appl Environ Microbiol. 1993 Oct;59(10):3438-46 PMID: 8250566
  35. The potential for intraspecific horizontal gene exchange by natural genetic transformation: sexual isolation among genomovars of Pseudomonas stutzeri.
    Microbiology. 2000 Dec;146 Pt 12:3081-90 PMID: 11101666
  36. Molecular genetic analysis of type-4 pilus biogenesis and twitching motility using Pseudomonas aeruginosa as a model system--a review.
    Gene. 1997 Jun 11;192(1):109-15 PMID: 9224880
  37. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes.
    Gene. 1995 Dec 1;166(1):175-6 PMID: 8529885
  38. Natural genetic transformation of Pseudomonas stutzeri by sand-adsorbed DNA.
    Arch Microbiol. 1990;154(4):380-5 PMID: 2244790
  39. Pseudomonas stutzeri and related species undergo natural transformation.
    J Bacteriol. 1983 Jan;153(1):93-9 PMID: 6571730
  40. Identification and characterization of novel competence genes comA and exbB involved in natural genetic transformation of Pseudomonas stutzeri.
    Res Microbiol. 2001 Jun;152(5):451-60 PMID: 11446513
  41. Isolation of a Pseudomonas stutzeri strain that degrades o-xylene.
    Appl Environ Microbiol. 1987 Sep;53(9):2129-32 PMID: 3674872
  42. Bacterial gene transfer by natural genetic transformation in the environment.
    Microbiol Rev. 1994 Sep;58(3):563-602 PMID: 7968924
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.68.2.865-873.2002
Published
2002-02-00
Pages
865-73
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC126724
Subset
IM
Databases
GENBANK
AJ270451, AJ270452, AJ270456, AJ312158, AJ312160, AJ312161, AJ312162, AJ312164, AJ312165, AJ312166, AJ312167, AJ312168, AJ312169, AJ312171, AJ312175, AJ319662
External Links
PubMed source DOI: 10.1128/AEM.68.2.865-873.2002 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2