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PMID: 11819844 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Establishment of cell clones with different metastatic potential from the metastatic hepatocellular carcinoma cell line MHCC97.

World journal of gastroenterology ·Vol. 7 ·No. 5 ·2001-10-00 ·Pages 630-6

Li Y, Tang ZY, Ye SL, Liu YK, Chen J, Xue Q, Chen J, Gao DM, Bao WH

Abstract

To establish clone cells with different metastatic potential for the study of metastasis-related mechanisms. Cloning procedure was performed on parental hepatocellular carcinoma (HCC) cell line MHCC97, and biological characteristics of the target clones selected by in vivo screening were studied. Two clones with high (MHCC97-H) and low (MHCC97-L) metastatic potential were isolated from the parent cell line. Compared with MHCC97-L, MHCC97-H had smaller cell size (average cell diameter 43 microm vs 50 microm) and faster in vitro and in vivo growth rate (tumor cell doubling time was 34.2h vs 60.0h). The main ranges of chromosomes were 55-58 in MHCC97-H and 57-62 in MHCC97-L. Boyden chamber in vitro invasion assay demonstrated that the number of penetrating cells through the artificial basement membrane was (37.5 +/- 11.0) cells/field for MHCC97-H vs (17.7 +/- 6.3)/field for MHCC97-L. The proportions of cells in G0-G1 phase, S phase, and G2-M phase for MHCC97-H/MHCC97-L were 0.56/0.65, 0.28/0.25 and 0.16/0.10, respectively, as measured by flow cytometry. The serum AFP levels in nude mice 5wk after orthotopic implantation of tumor tissue were (246 +/- 66) microg.L(-1) for MHCC97-H and (91 +/- 66) microg.L(-1) for MHCC97-L. The pulmonary metastatic rate was 100% (10/10) vs 40% (4/10). Two clones of the same genetic background but with different biological behaviors were established, which could be valuable models for investigation on HCC metastasis.

MeSH Terms
Albumins/analysis Animals Carcinoma, Hepatocellular/genetics,secondary Cell Division Chromosomes Clone Cells Flow Cytometry Hepatitis B/pathology Hepatitis B Surface Antigens/analysis Hepatitis B virus/isolation & purification Humans Keratins/analysis Liver/pathology Liver Neoplasms, Experimental/genetics,secondary Male Mice Mice, Inbred BALB C Mice, Nude Neoplasm Invasiveness Tumor Cells, Cultured/chemistry,cytology,virology Virus Integration alpha-Fetoproteins/analysis
Chemicals
Albumins Hepatitis B Surface Antigens alpha-Fetoproteins Keratins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Li Y
Liver Cancer Institute & Zhongshan Hospital of Fudan University, 136 Yixueyuan Road, Shanghai 200032,China.
Tang Z Y
Ye S L
Liu Y K
Chen J
Xue Q
Chen J
Gao D M
Bao W H
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Article Info
Journal
World journal of gastroenterology
Abbr.
World J Gastroenterol
ISSN
1007-9327
DOI
10.3748/wjg.v7.i5.630
Published
2001-10-00
Pages
630-6
Language
English
Region
United States
NLM ID
100883448
PMCID
PMC4695564
Subset
IM
Corrections
CommentIn
PMID: 11854899
External Links
PubMed source DOI: 10.3748/wjg.v7.i5.630 PMC Full Text View journal details
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