Establishment of a clonal strain of hepatoma cells which maintain in culture the five enzymes of the urea cycle.

Ammonia Animals Arginase/metabolism Arginine Bucladesine/pharmacology Carbamates Carcinoma, Hepatocellular/enzymology,pathology Chromosomes Clone Cells Epithelium/enzymology Glucagon/pharmacology Hydrocortisone/pharmacology Ligases/metabolism Liver Neoplasms Lyases/metabolism Microscopy, Phase-Contrast Neoplasms, Experimental/enzymology Organophosphorus Compounds Ornithine Carbamoyltransferase/metabolism Phosphotransferases/metabolism Rats Succinates Time Factors Tyrosine Transaminase/metabolism Urea/metabolism

Carbamates Organophosphorus Compounds Succinates Bucladesine Ammonia Urea Glucagon Arginine Ornithine Carbamoyltransferase Tyrosine Transaminase Phosphotransferases Arginase Lyases Ligases Hydrocortisone