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PMID: 11784788 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multipotent neural stem cells reside into the rostral extension and olfactory bulb of adult rodents.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 22 ·No. 2 ·2002-01-15 ·Pages 437-45

Gritti A, Bonfanti L, Doetsch F, Caille I, Alvarez-Buylla A, Lim DA, Galli R, Verdugo JM, Herrera DG, Vescovi AL

Abstract

The lateral walls of the forebrain lateral ventricles are the richest source of stem cells in the adult mammalian brain. These stem cells give rise to new olfactory neurons that are renewed throughout life. The neurons originate in the subventricular zone (SVZ), migrate within the rostral extension (RE) of the SVZ along the rostral migratory stream (RMS) within tube-like structures formed of glial cells, to eventually reach the olfactory bulb (OB). We demonstrate that, contrary to the current view, multipotential (neuronal-astroglial-oligodendroglial) precursors with stem cell features can be isolated not only from the SVZ but also from the entire RE, including the distal portion within the OB. Specifically, these stem cells do not derive from the migratory neuroblasts coming from the SVZ. Interestingly, stem cells isolated from the proximal RE generate significantly more oligodendrocytes, and those from the distal RE proliferate significantly more slowly than stem cells derived from the SVZ and other RE regions. These findings demonstrate that stem cells are not confined to the forebrain periventricular region and indicate that stem cells endowed with different functional characteristics occur at different levels of the SVZ-RE pathway.

MeSH Terms
Animals Astrocytes/cytology Cell Culture Techniques/methods Cell Differentiation/physiology Cell Division/drug effects Cell Line/cytology Cell Movement/physiology Cell Separation Cells, Cultured Clone Cells/classification,cytology,drug effects Growth Substances/pharmacology Lateral Ventricles/cytology Mice Neurons/classification,cytology,metabolism Neurotransmitter Agents/metabolism Olfactory Bulb/cytology Oligodendroglia/cytology Phenotype Spheroids, Cellular/cytology Stem Cells/classification,cytology,drug effects Time Factors
Chemicals
Growth Substances Neurotransmitter Agents
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Gritti Angela
Institute for Stem Cell Research, Department of Biotechnology, San Raffaele Hospital, 20132 Milan, Italy. gritti.angela@hsr.it.
Bonfanti Luca
Doetsch Fiona
Caille Isabelle
Alvarez-Buylla Arturo
Lim Daniel A
Galli Rossella
Verdugo Jose Manuel Garcia
Herrera Daniel G
Vescovi Angelo L
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
Email
gritti.angela@hsr.it
Published
2002-01-15
Pages
437-45
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6758684
Subset
IM
Grants
Telethon · E.1246 · Italy
External Links
PubMed source PMC Full Text View journal details
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