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PMID: 11782448 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore.

Genes & development ·Vol. 16 ·No. 1 ·2002-01-01 ·Pages 101-13

Measday V, Hailey DW, Pot I, Givan SA, Hyland KM, Cagney G, Fields S, Davis TN, Hieter P

Abstract

The budding yeast kinetochore is composed of an inner and outer protein complex, which binds to centromere (CEN) DNA and attaches to microtubules. We performed a genetic synthetic dosage lethality screen to identify novel kinetochore proteins in a collection of chromosome transmission fidelity mutants. Our screen identified several new kinetochore-related proteins including YLR381Wp/Ctf3p, which is a member of a conserved family of centromere-binding proteins. Ctf3p interacts with Mcm22p, Mcm16p, and the outer kinetochore protein Ctf19p. We used chromatin immunoprecipitation to demonstrate that Ctf3p, Mcm22p, and Mcm16p bind to CEN DNA in a Ctf19p-dependent manner. In addition, Ctf3p, Mcm22p, and Mcm16p have a localization pattern similar to other kinetochore proteins. The fission yeast Ctf3p homolog, Mis6, is required for loading of a CENP-A centromere specific histone, Cnp1, onto centromere DNA. We find however that Ctf3p is not required for loading of the budding yeast CENP-A homolog, Cse4p, onto CEN DNA. In contrast, Ctf3p and Ctf19p fail to bind properly to the centromere in a cse4-1 mutant strain. We conclude that the requirements for CENP-A loading onto centromere DNA differ in fission versus budding yeast.

MeSH Terms
Amino Acid Sequence Cell Cycle Proteins Chromosome Segregation/physiology DNA-Binding Proteins Fungal Proteins/physiology Kinetochores/physiology Molecular Sequence Data Mutation Saccharomyces cerevisiae/physiology Saccharomyces cerevisiae Proteins/physiology Schizosaccharomyces pombe Proteins Sequence Homology, Amino Acid
Chemicals
Cell Cycle Proteins Ctf3 protein, S cerevisiae DNA-Binding Proteins Fungal Proteins MCM16 protein, S cerevisiae MCM22 protein, S cerevisiae Mis6 protein, S pombe Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Measday Vivien
Department of Medical Genetics, University of British Columbia, Vancouver, BC V5Z 4H4, Canada.
Hailey Dale W
Pot Isabelle
Givan Scott A
Hyland Katherine M
Cagney Gerard
Fields Stan
Davis Trisha N
Hieter Philip
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.949302
Published
2002-01-01
Pages
101-13
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC155308
Subset
IM
Grants
NCI NIH HHS · P01 CA016519 · United States
NCRR NIH HHS · P41 RR011823 · United States
NCI NIH HHS · CA16519 · United States
NCRR NIH HHS · RR11823 · United States
External Links
PubMed source DOI: 10.1101/gad.949302 PMC Full Text View journal details
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