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PMID: 1177864 Published · ppublish English Journal Article

The synthetase gene of the RNA phages R17, MS2 and f2 has a single UAG terminator codon.

Molecular & general genetics : MGG ·Vol. 139 ·No. 1 ·1975-08-05 ·Pages 19-31

Atkins JF, Gesteland RF

Abstract

Translation of the RNA from the wild-type bacteriophages R17, MS2, and f2 in bacterial cell-free extracts containing an amber suppressor yields 30-40% of the synthetase with an approximate molecular weight of 63 500, slightly larger than the major synthetase product (63 000 daltons). The occurrence of the 63 500 dalton in vitro product is dependent on the presence of an amber suppressor, and we predict that it is due to read-through of a UAG termination codon at the end of the synthetase gene. Previous results of Capecchi and Klein (Nature, 226, 1029-1033, 1070) showed that antibodies to both release factors RF1 and RF2 are required to block release of synthetase, suggesting that synthetase is released at a UAA codon. If the interpretations of both experiments are correct, the termination and release may not be synonomous and may be spatially separated. In addition there is the unexplained fact that 7% of the synthetase made in vitro in both su+ and su- extracts with either R17, MS2 or f2 as template has an apparent molecular weight of 66 000.

MeSH Terms
Autoradiography Codon Coliphages Enzymes Genes Methionine Molecular Weight Protein Biosynthesis RNA Nucleotidyltransferases RNA, Messenger Sulfur Radioisotopes Suppression, Genetic Viral Proteins/analysis Virus Replication
Chemicals
Codon Enzymes RNA, Messenger Sulfur Radioisotopes Viral Proteins Methionine RNA Nucleotidyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Atkins J F
Gesteland R F
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39 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267992
Published
1975-08-05
Pages
19-31
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267992
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