Home LiteratureArticle Details
PMID: 11773130 Published · ppublish English Evaluation Study Journal Article Research Support, Non-U.S. Gov't

Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa.

Journal of clinical microbiology ·Vol. 40 ·No. 1 ·2002-01-00 ·Pages 271-4

Paton AW, Paton JC

Abstract

We recently described a novel megaplasmid-encoded adhesin produced by certain Shiga toxigenic Escherichia coli (STEC) strains that lack the locus for enterocyte effacement (LEE) pathogenicity island. This adhesin, designated Saa (STEC autoagglutinating adhesin), may be a marker for a subset of LEE-negative STEC strains capable of causing severe gastrointestinal and systemic diseases in humans. In this study, we developed a pentavalent PCR assay for the detection of saa as well as other proven and putative STEC virulence genes (stx1, stx2, eae, and ehxA). The five primer pairs used in the assay do not interfere with each other and generate amplification products of 119, 180, 255, 384, and 534 bp.

MeSH Terms
Adhesins, Bacterial/genetics DNA Primers Escherichia coli/genetics,metabolism,pathogenicity Escherichia coli Infections/diagnosis,microbiology Escherichia coli Proteins/genetics Feces/microbiology Humans Polymerase Chain Reaction/methods Sensitivity and Specificity Shiga Toxins/genetics,metabolism Virulence/genetics
Chemicals
Adhesins, Bacterial DNA Primers Escherichia coli Proteins SAA protein, E coli Shiga Toxins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Paton Adrienne W
Department of Molecular Biosciences, Adelaide University, Adelaide, South Australia 5005, Australia.
Paton James C
References (22)
22 references, click to expand
  1. Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infections.
    J Infect Dis. 1989 Dec;160(6):994-8 PMID: 2685131
  2. Infection by verocytotoxin-producing Escherichia coli.
    Clin Microbiol Rev. 1989 Jan;2(1):15-38 PMID: 2644022
  3. Cloning and characterization of the eae gene of enterohaemorrhagic Escherichia coli O157:H7.
    Mol Microbiol. 1992 Feb;6(3):411-7 PMID: 1552854
  4. Rapid and sensitive method for detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction.
    Appl Environ Microbiol. 1992 Dec;58(12):3809-15 PMID: 1476425
  5. Detection and characterization of the eae gene of Shiga-like toxin-producing Escherichia coli using polymerase chain reaction.
    J Clin Microbiol. 1993 May;31(5):1268-74 PMID: 8501228
  6. Virulence of enterohemorrhagic Escherichia coli O91:H21 clinical isolates in an orally infected mouse model.
    Infect Immun. 1993 Sep;61(9):3832-42 PMID: 8359904
  7. Direct detection of Escherichia coli Shiga-like toxin genes in primary fecal cultures by polymerase chain reaction.
    J Clin Microbiol. 1993 Nov;31(11):3063-7 PMID: 8263203
  8. Sequence heterogeneity of the eae gene and detection of verotoxin-producing Escherichia coli using serotype-specific primers.
    Epidemiol Infect. 1994 Jun;112(3):449-61 PMID: 8005211
  9. Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933.
    Infect Immun. 1995 Mar;63(3):1055-61 PMID: 7868227
  10. Direct detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction.
    Mol Cell Probes. 1995 Aug;9(4):259-64 PMID: 7477022
  11. Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157.
    J Clin Microbiol. 1998 Feb;36(2):598-602 PMID: 9466788
  12. The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69.
    Mol Microbiol. 1998 Apr;28(1):1-4 PMID: 9593291
  13. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections.
    Clin Microbiol Rev. 1998 Jul;11(3):450-79 PMID: 9665978
  14. Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7.
    Infect Immun. 1998 Aug;66(8):3810-7 PMID: 9673266
  15. Associations between virulence factors of Shiga toxin-producing Escherichia coli and disease in humans.
    J Clin Microbiol. 1999 Mar;37(3):497-503 PMID: 9986802
  16. Molecular characterization of a Shiga toxigenic Escherichia coli O113:H21 strain lacking eae responsible for a cluster of cases of hemolytic-uremic syndrome.
    J Clin Microbiol. 1999 Oct;37(10):3357-61 PMID: 10488206
  17. Direct detection of Shiga toxigenic Escherichia coli strains belonging to serogroups O111, O157, and O113 by multiplex PCR.
    J Clin Microbiol. 1999 Oct;37(10):3362-5 PMID: 10488207
  18. Interactions between enteropathogenic Escherichia coli and host epithelial cells.
    Trends Microbiol. 1997 Mar;5(3):109-14 PMID: 9080609
  19. EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V.
    Mol Microbiol. 1997 May;24(4):767-78 PMID: 9194704
  20. Diarrheagenic Escherichia coli.
    Clin Microbiol Rev. 1998 Jan;11(1):142-201 PMID: 9457432
  21. Characterization of Saa, a novel autoagglutinating adhesin produced by locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains that are virulent for humans.
    Infect Immun. 2001 Nov;69(11):6999-7009 PMID: 11598075
  22. Haemolytic uraemic syndromes in the British Isles, 1985-8: association with verocytotoxin producing Escherichia coli. Part 2: Microbiological aspects.
    Arch Dis Child. 1990 Jul;65(7):722-7 PMID: 2201262
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.40.1.271-274.2002
Published
2002-01-00
Pages
271-4
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC120136
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.40.1.271-274.2002 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2