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PMID: 11756541 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Replication initiation patterns in the beta-globin loci of totipotent and differentiated murine cells: evidence for multiple initiation regions.

Molecular and cellular biology ·Vol. 22 ·No. 2 ·2002-01-00 ·Pages 442-52

Aladjem MI, Rodewald LW, Lin CM, Bowman S, Cimbora DM, Brody LL, Epner EM, Groudine M, Wahl GM

Abstract

The replication initiation pattern of the murine beta-globin locus was analyzed in totipotent embryonic stem cells and in differentiated cell lines. Initiation events in the murine beta-globin locus were detected in a region extending from the embryonic Ey gene to the adult betaminor gene, unlike the restricted initiation observed in the human locus. Totipotent and differentiated cells exhibited similar initiation patterns. Deletion of the region between the adult globin genes did not prevent initiation in the remainder of the locus, suggesting that the potential to initiate DNA replication was not contained exclusively within the primary sequence of the deleted region. In addition, a deletion encompassing the six identified 5' hypersensitive sites in the mouse locus control region had no effect on initiation from within the locus. As this deletion also did not affect the chromatin structure of the locus, we propose that the sequences determining both chromatin structure and replication initiation lie outside the hypersensitive sites removed by the deletion.

MeSH Terms
Animals Base Sequence Binding Sites/genetics Cell Differentiation Cell Line DNA/genetics DNA Replication Gene Expression Regulation Globins/genetics Humans Mice S Phase Sequence Deletion Stem Cells/cytology,metabolism
Chemicals
Globins DNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Aladjem Mirit I
Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, Maryland 20892-4255, USA. maladjem@helix.nih.gov
Rodewald Luo Wei
Lin Chii Mai
Bowman Sarah
Cimbora Daniel M
Brody Linnea L
Epner Elliot M
Groudine Mark
Wahl Geoffrey M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.22.2.442-452.2002
Email
maladjem@helix.nih.gov
Published
2002-01-00
Pages
442-52
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC139749
Subset
IM
Grants
NIDDK NIH HHS · DK44746 · United States
NCI NIH HHS · R01 CA048405 · United States
NIDDK NIH HHS · R37 DK044746 · United States
NCI NIH HHS · CA48405 · United States
NIGMS NIH HHS · GM51104 · United States
External Links
PubMed source DOI: 10.1128/MCB.22.2.442-452.2002 PMC Full Text View journal details
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