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PMID: 11739793 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Schizosaccharomyces pombe spo3+ gene is required for assembly of the forespore membrane and genetically interacts with psy1(+)-encoding syntaxin-like protein.

Molecular biology of the cell ·Vol. 12 ·No. 12 ·2001-12-00 ·Pages 3955-72

Nakamura T, Nakamura-Kubo M, Hirata A, Shimoda C

Abstract

Formation of the forespore membrane, which becomes the plasma membrane of spores, is an intriguing step in the sporulation of the fission yeast Schizosaccharomyces pombe. Here we report two novel proteins that localize to the forespore membrane. spo3(+) encodes a potential membrane protein, which was expressed only during sporulation. Green fluorescent protein (GFP) fusion revealed that Spo3 localized to the forespore membrane. The spo3 disruptant was viable and executed meiotic nuclear divisions as efficiently as the wild type but did not form spores. One of the spo3 alleles, spo3-KC51, was dose-dependently suppressed by psy1(+), which encodes a protein similar to mammalian syntaxin-1A, a component of the plasma membrane docking/fusion complex. psy1(+) was essential for vegetative growth, and its transcription was enhanced during sporulation. As expected, Psy1 localized to the plasma membrane during vegetative growth. Interestingly, Psy1 on the plasma membrane disappeared immediately after first meiotic division and relocalized to the forespore membrane as the second division initiated. In the spo3 null mutant, the forespore membrane was initiated but failed to develop a normal morphology. Electron microscopy revealed that membrane vesicles were accumulated in the cytoplasm of immature spo3Delta asci. These results suggest that Spo3 is a key component of the forespore membrane and is essential for its assembly acting in collaboration with the syntaxin-like protein.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Western Cell Division Cell Membrane/metabolism Gene Expression Regulation, Fungal Meiosis Membrane Proteins/chemistry,genetics,metabolism Microscopy, Electron Microscopy, Fluorescence Models, Biological Molecular Sequence Data Qa-SNARE Proteins RNA, Messenger/metabolism Schizosaccharomyces/cytology,genetics,growth & development,ultrastructure Schizosaccharomyces pombe Proteins/chemistry,genetics,metabolism Sequence Homology, Amino Acid Spores, Fungal/cytology,genetics,growth & development,ultrastructure Suppression, Genetic/genetics Time Factors
Chemicals
Membrane Proteins Qa-SNARE Proteins RNA, Messenger Schizosaccharomyces pombe Proteins Spo3 protein, S pombe
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nakamura T
Department of Biology, Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.
Nakamura-Kubo M
Hirata A
Shimoda C
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
Published
2001-12-00
Pages
3955-72
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC60768
Subset
IM
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