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PMID: 11705982 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the adhesin of Escherichia coli F18 fimbriae.

Infection and immunity ·Vol. 69 ·No. 12 ·2001-12-00 ·Pages 7941-5

Smeds A, Hemmann K, Jakava-Viljanen M, Pelkonen S, Imberechts H, Palva A

Abstract

Previous research has suggested that the adhesin encoded by the F18 fimbrial operon in Escherichia coli is either the FedE or FedF protein. In this work, we show that anti-FedF antibodies, unlike anti-FedE serum, were able to inhibit E. coli adhesion to porcine enterocytes. Moreover, specific adhesion to enterocytes was shown with purified FedF-maltose binding protein.

MeSH Terms

Adhesins, Bacterial Animals Antibodies, Bacterial Bacterial Adhesion Cloning, Molecular Enterocytes/microbiology Escherichia coli/genetics,pathogenicity Escherichia coli Proteins/immunology,physiology Fimbriae, Bacterial/chemistry,genetics Fluorescent Antibody Technique, Indirect Ileum/cytology,microbiology Jejunum/cytology,microbiology Molecular Sequence Data Recombinant Fusion Proteins Swine

Chemicals

Adhesins, Bacterial Antibodies, Bacterial Escherichia coli Proteins FedF protein, E coli Recombinant Fusion Proteins

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Smeds A

Faculty of Veterinary Medicine, Department of Basic Veterinary Sciences, Section of Microbiology, 00014 University of Helsinki, Finland.

Hemmann K

Jakava-Viljanen M

Pelkonen S

Imberechts H

Palva A

References (12)

12 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/IAI.69.12.7941-7945.2001

Published

2001-12-00

Pages

7941-5

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC98896

Subset

Databases

GENBANK

AF222806

External Links

PubMed source DOI: 10.1128/IAI.69.12.7941-7945.2001 PMC Full Text View journal details

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Characterization of the adhesin of Escherichia coli F18 fimbriae.

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