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PMID: 11689606 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of limiting homology at the site of intermolecular recombinogenic template switching during Moloney murine leukemia virus replication.

Journal of virology ·Vol. 75 ·No. 23 ·2001-12-00 ·Pages 11263-74

Pfeiffer JK, Telesnitsky A

Abstract

A Moloney murine leukemia virus-based single-replication-cycle assay was developed to study the effects of limiting the extent of template and primer strand complementarity on recombinogenic template switching. This system mimicked forced copy choice recombination in which nascent DNA transfers from the end of a donor template to an acceptor position on the other copackaged RNA. When acceptor target regions with different extents of complementarity to the transferring DNA were tested, efficient recombination occurred with as few as 14 complementary nucleotides. The frequencies of correct targeting, transfer-associated errors, mismatch extension, and transfer before reaching the end of the donor template were determined. All four molecular events occurred, with their proportions varying depending on the nature of acceptor/transferring DNA complementarity. When complementarity was severely limited, recombination was inefficient and most products resulted from aberrant second-strand transfer rather than from forced template switching between RNAs. Other classes of reverse transcription products, including some that resulted from template switching between virus and host sequences, were also observed when homology between the acceptor and donor was limited.

MeSH Terms
3T3 Cells Animals Base Pair Mismatch Base Sequence DNA, Viral/genetics Mice Molecular Sequence Data Moloney murine leukemia virus/genetics,physiology RNA, Viral/genetics Recombination, Genetic Sequence Homology, Nucleic Acid Templates, Genetic Transcription, Genetic Virus Replication
Chemicals
DNA, Viral RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pfeiffer J K
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0620, USA.
Telesnitsky A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.75.23.11263-11274.2001
Published
2001-12-00
Pages
11263-74
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC114711
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.75.23.11263-11274.2001 PMC Full Text View journal details
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