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PMID: 11585527 Published · ppublish English Journal Article

Partial genetic characterization of West Nile virus strains, New York State, 2000.

Emerging infectious diseases ·Vol. 7 ·No. 4 ·2001-00-00 ·Pages 650-3

Ebel GD, Dupuis AP, Ngo K, Nicholas D, Kauffman E, Jones SA, Young D, Maffei J, Shi PY, Bernard K, Kramer LD

Abstract

We analyzed nucleotide sequences from the envelope gene of 11 West Nile (WN) virus strains collected in New York State during the 2000 transmission season to determine whether they differed genetically from each other and from the initial strain isolated in 1999. The complete envelope genes of these strains were amplified by reverse transcription-polymerase chain reaction. The resulting sequences were aligned, the genetic distances were computed, and a phylogenetic tree was constructed. Ten (0.7%) of 1,503 positions in the envelope gene were polymorphic in one or more sequences. The genetic distances were 0.003 or less. WN virus strains circulating in 2000 were homogeneous with respect to one another and to a strain isolated in 1999.

MeSH Terms
Animals Bird Diseases/epidemiology,virology Culex/virology Disease Reservoirs/veterinary Humans Insect Vectors/virology New York/epidemiology Songbirds/virology Viral Envelope Proteins/genetics West Nile Fever/epidemiology,veterinary,virology West Nile virus/classification,genetics,isolation & purification
Chemicals
Viral Envelope Proteins
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Ebel G D
Arbovirus Laboratories, New York State Department of Health, 5668 State Farm Road, Slingerlands, NY 12159, USA. ebel@wadsworth.org
Dupuis A P
Ngo K
Nicholas D
Kauffman E
Jones S A
Young D
Maffei J
Shi P Y
Bernard K
Kramer L D
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Article Info
Journal
Emerging infectious diseases
Abbr.
Emerg Infect Dis
ISSN
1080-6040
Published
2001-00-00
Pages
650-3
Language
English
Region
United States
NLM ID
9508155
PMCID
PMC2631742
Subset
IM
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