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PMID: 11517328 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein-Barr virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 18 ·2001-08-28 ·Pages 10085-9

Chaudhuri B, Xu H, Todorov I, Dutta A, Yates JL

Abstract

The 165-kb chromosome of Epstein-Barr virus (EBV) is replicated by cellular enzymes only once per cell cycle in human cells that are latently infected. Here, we report that the human origin recognition complex, ORC, can be detected in association with an EBV replication origin, oriP, in cells by using antibodies against three different subunits of human ORC to precipitate crosslinked chromatin. Mcm2, a subunit of the MCM replication licensing complex, was found to associate with oriP during G(1) and to dissociate from it during S phase. The detection of ORC and Mcm2 at oriP was shown to require the presence of the 120-bp replicator of oriP. Licensing and initiation of replication at oriP of EBV thus seem to be mediated by ORC. This is an example of a virus apparently using ORC and associated factors for the propagation of its genome.

MeSH Terms
Cell Cycle Cell Line DNA Replication DNA-Binding Proteins/metabolism Herpesvirus 4, Human/genetics,metabolism,physiology Humans Minichromosome Maintenance Complex Component 2 Nuclear Proteins/metabolism Origin Recognition Complex Replication Origin Virus Replication
Chemicals
DNA-Binding Proteins Nuclear Proteins OriP-binding protein 1 Origin Recognition Complex Minichromosome Maintenance Complex Component 2
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chaudhuri B
Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
Xu H
Todorov I
Dutta A
Yates J L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.181347998
Published
2001-08-28
Epub
2001-00-21
Pages
10085-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC56919
Subset
IM
Grants
NCI NIH HHS · P30 CA016056 · United States
NCI NIH HHS · R01 CA060499 · United States
NCI NIH HHS · CA16056 · United States
NCI NIH HHS · CA14322 · United States
NCI NIH HHS · CA60499 · United States
External Links
PubMed source DOI: 10.1073/pnas.181347998 PMC Full Text View journal details
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