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PMID: 11509664 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Xenopus U3 snoRNA GAC-Box A' and Box A sequences play distinct functional roles in rRNA processing.

Molecular and cellular biology ·Vol. 21 ·No. 18 ·2001-09-00 ·Pages 6210-21

Borovjagin AV, Gerbi SA

Abstract

Mutations in the 5' portion of Xenopus U3 snoRNA were tested for function in oocytes. The results revealed a new cleavage site (A0) in the 3' region of vertebrate external transcribed spacer sequences. In addition, U3 mutagenesis uncoupled cleavage at sites 1 and 2, flanking the 5' and 3' ends of 18S rRNA, and generated novel intermediates: 19S and 18.5S pre-rRNAs. Furthermore, specific nucleotides in Xenopus U3 snoRNA that are required for cleavages in pre-rRNA were identified: box A is essential for site A0 cleavage, the GAC-box A' region is necessary for site 1 cleavage, and the 3' end of box A' and flanking nucleotides are required for site 2 cleavage. Differences between metazoan and yeast U3 snoRNA-mediated rRNA processing are enumerated. The data support a model where metazoan U3 snoRNA acts as a bridge to draw together the 5' and 3' ends of the 18S rRNA coding region within pre-rRNA to coordinate their cleavage.

MeSH Terms
Animals Base Sequence Gene Expression Regulation Molecular Sequence Data RNA Processing, Post-Transcriptional RNA, Ribosomal/genetics,metabolism RNA, Small Nucleolar/genetics,metabolism Sequence Analysis, RNA Xenopus laevis/genetics,metabolism
Chemicals
RNA, Ribosomal RNA, Small Nucleolar RNA, U3 small nucleolar
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Borovjagin A V
Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912, USA.
Gerbi S A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.18.6210-6221.2001
Published
2001-09-00
Pages
6210-21
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC87338
Subset
IM
Grants
NIGMS NIH HHS · R01 GM061945 · United States
NIGMS NIH HHS · GM 61945 · United States
External Links
PubMed source DOI: 10.1128/MCB.21.18.6210-6221.2001 PMC Full Text View journal details
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