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PMID: 10397754 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Role of the box C/D motif in localization of small nucleolar RNAs to coiled bodies and nucleoli.

Molecular biology of the cell ·Vol. 10 ·No. 7 ·1999-07-00 ·Pages 2131-47

Narayanan A, Speckmann W, Terns R, Terns MP

Abstract

Small nucleolar RNAs (snoRNAs) are a large family of eukaryotic RNAs that function within the nucleolus in the biogenesis of ribosomes. One major class of snoRNAs is the box C/D snoRNAs named for their conserved box C and box D sequence elements. We have investigated the involvement of cis-acting sequences and intranuclear structures in the localization of box C/D snoRNAs to the nucleolus by assaying the intranuclear distribution of fluorescently labeled U3, U8, and U14 snoRNAs injected into Xenopus oocyte nuclei. Analysis of an extensive panel of U3 RNA variants showed that the box C/D motif, comprised of box C', box D, and the 3' terminal stem of U3, is necessary and sufficient for the nucleolar localization of U3 snoRNA. Disruption of the elements of the box C/D motif of U8 and U14 snoRNAs also prevented nucleolar localization, indicating that all box C/D snoRNAs use a common nucleolar-targeting mechanism. Finally, we found that wild-type box C/D snoRNAs transiently associate with coiled bodies before they localize to nucleoli and that variant RNAs that lack an intact box C/D motif are detained within coiled bodies. These results suggest that coiled bodies play a role in the biogenesis and/or intranuclear transport of box C/D snoRNAs.

MeSH Terms
Animals Base Sequence Cell Nucleolus/genetics Conserved Sequence Fluorescein/chemistry Molecular Sequence Data Mutation Nucleic Acid Conformation Oocytes Organelles/genetics RNA, Small Nuclear/chemistry,genetics,metabolism Temperature Xenopus
Chemicals
RNA, Small Nuclear Fluorescein
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Narayanan A
Department of Genetics, University of Georgia, Athens, Georgia 30602, USA.
Speckmann W
Terns R
Terns M P
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.10.7.2131
Published
1999-07-00
Pages
2131-47
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC25425
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.10.7.2131 PMC Full Text View journal details
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