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PMID: 11488992 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Effects of hyaluronan on the invasive properties of human breast cancer cells in vitro.

International journal of experimental pathology ·Vol. 82 ·No. 3 ·2001-06-00 ·Pages 193-200

Herrera-Gayol A, Jothy S

Abstract

Hyaluronan (HA) is a high molecular weight glycosaminoglycan present mostly in the extracellular matrix (ECM). HA binds to specific receptors such as CD44. Its production is increased at the tumour-stroma interface, including those in breast cancer tumours. It has been suggested that it facilitates invasion of tumour cells into the ECM by a hydrodynamic effect, or by altering tumour cell behaviour. Using in vitro tests we studied the effect of immobilized (iHA) and soluble (sHA) HA on the invasive properties of four human breast cancer cell lines with different levels of CD44 expression. Our results show that iHA acts as an adhesive, haptotactic, and motility stimulating factor for the CD44 positive Hs578T cells and induces the expression of membrane CD44. sHA also changes the motility properties of the Hs578T and MDA-231 cells and increases their CD44 expression. sHA or iHA have no measurable effect on the adhesion, motility or CD44 expression of the ZR-75-1 and MCF-7 breast cancer cells. Our results establish that in high CD44 expressing breast cancer cells HA modulates tumour cell adhesion and motility and also increases the expression of its own receptor, CD44.

MeSH Terms
Analysis of Variance Breast Neoplasms/immunology,pathology Cell Adhesion/drug effects Cell Membrane/drug effects,metabolism Cell Movement/drug effects Female Humans Hyaluronan Receptors/metabolism Hyaluronic Acid/pharmacology Neoplasm Invasiveness Protein Binding Tumor Cells, Cultured
Chemicals
Hyaluronan Receptors Hyaluronic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Herrera-Gayol A
Department of Pathology, McGill University, Montreal, Quebec, Canada.
Jothy S
References (27)
27 references, click to expand
  1. Hyaluronic acid induces tumour necrosis factor-alpha production by human macrophages in vitro.
    Br J Plast Surg. 1997 Jul;50(5):362-8 PMID: 9245871
  2. CD44H regulates tumor cell migration on hyaluronate-coated substrate.
    J Cell Biol. 1992 Aug;118(4):971-7 PMID: 1380003
  3. Migration of highly aggressive melanoma cells on hyaluronic acid is associated with functional changes, increased turnover and shedding of CD44 receptors.
    J Cell Sci. 1996 Jul;109 ( Pt 7):1957-64 PMID: 8832418
  4. Binding and degradation of hyaluronan by human breast cancer cell lines expressing different forms of CD44: correlation with invasive potential.
    J Cell Physiol. 1994 Aug;160(2):275-86 PMID: 7518822
  5. Migration of human melanoma cells on hyaluronate is related to CD44 expression.
    J Invest Dermatol. 1993 Feb;100(2):115-20 PMID: 8429233
  6. Molecular and cellular analysis of basement membrane invasion by human breast cancer cells in Matrigel-based in vitro assays.
    Breast Cancer Res Treat. 1993;24(3):241-55 PMID: 8435479
  7. Hyaluronan as a propellant for epithelial movement: the development of semicircular canals in the inner ear of Xenopus.
    Development. 1991 Jun;112(2):541-50 PMID: 1794322
  8. Association of increased basement membrane invasiveness with absence of estrogen receptor and expression of vimentin in human breast cancer cell lines.
    J Cell Physiol. 1992 Mar;150(3):534-44 PMID: 1537883
  9. Localization of hyaluronan in normal breast tissue, radial scar, and tubular breast carcinoma.
    Hum Pathol. 1993 Dec;24(12):1294-7 PMID: 8276376
  10. Hyaluronan fragments activate an NF-kappa B/I-kappa B alpha autoregulatory loop in murine macrophages.
    J Exp Med. 1996 May 1;183(5):2373-8 PMID: 8642348
  11. Angiogenic oligosaccharides of hyaluronan induce protein tyrosine kinase activity in endothelial cells and activate a cytoplasmic signal transduction pathway resulting in proliferation.
    Lab Invest. 1998 Aug;78(8):987-1003 PMID: 9714186
  12. Early-response gene signalling is induced by angiogenic oligosaccharides of hyaluronan in endothelial cells. Inhibition by non-angiogenic, high-molecular-weight hyaluronan.
    Int J Cancer. 1997 Apr 10;71(2):251-6 PMID: 9139851
  13. Angiogenesis induced by degradation products of hyaluronic acid.
    Science. 1985 Jun 14;228(4705):1324-6 PMID: 2408340
  14. The effect of hyaluronate and its oligosaccharides on endothelial cell proliferation and monolayer integrity.
    Exp Cell Res. 1989 Jul;183(1):179-96 PMID: 2472284
  15. CD44 variant isoform CD44v10 expression of human melanoma cell lines is upregulated by hyaluronate and correlates with migration.
    Melanoma Res. 1999 Jun;9(3):223-31 PMID: 10465577
  16. Differential synthesis and binding of hyaluronan by human breast cancer cell lines.
    Oncol Rep. 1996 Nov;3(6):1011-6 PMID: 21594498
  17. Hyaluronan and cell locomotion.
    Cancer Metastasis Rev. 1992 Mar;11(1):21-30 PMID: 1380898
  18. Hyaluronan fragments induce nitric-oxide synthase in murine macrophages through a nuclear factor kappaB-dependent mechanism.
    J Biol Chem. 1997 Mar 21;272(12):8013-8 PMID: 9065473
  19. Hyaluronan (hyaluronic acid) and hyaluronectin in the extracellular matrix of human breast carcinomas: comparison between invasive and non-invasive areas.
    Int J Cancer. 1992 Aug 19;52(1):1-6 PMID: 1379993
  20. CD44 is the principal cell surface receptor for hyaluronate.
    Cell. 1990 Jun 29;61(7):1303-13 PMID: 1694723
  21. Hyaluronan and angiogenesis.
    Ciba Found Symp. 1989;143:187-201; discussion 201-7, 281-5 PMID: 2478344
  22. HA receptors: regulators of signalling to the cytoskeleton.
    J Cell Biochem. 1996 Jun 15;61(4):569-77 PMID: 8806080
  23. Differentiation state and invasiveness of human breast cancer cell lines.
    Breast Cancer Res Treat. 1994;31(2-3):325-35 PMID: 7881109
  24. Hyaluronan (HA) fragments induce chemokine gene expression in alveolar macrophages. The role of HA size and CD44.
    J Clin Invest. 1996 Nov 15;98(10):2403-13 PMID: 8941660
  25. Modulation of cell migration by integrin-mediated cytoskeletal linkages and ligand-binding affinity.
    J Cell Biol. 1996 Sep;134(6):1551-62 PMID: 8830782
  26. Regulation of hyaluronan-induced chemokine gene expression by IL-10 and IFN-gamma in mouse macrophages.
    J Immunol. 1998 Mar 15;160(6):3023-30 PMID: 9510207
  27. CD44 modulates Hs578T human breast cancer cell adhesion, migration, and invasiveness.
    Exp Mol Pathol. 1999 Apr;66(1):99-108 PMID: 10331969
Article Info
Journal
International journal of experimental pathology
Abbr.
Int J Exp Pathol
ISSN
0959-9673
DOI
10.1046/j.1365-2613.2001.iep0082-0193-x
Published
2001-06-00
Pages
193-200
Language
English
Region
England
NLM ID
9014042
PMCID
PMC2517708
Subset
IM
External Links
PubMed source DOI: 10.1046/j.1365-2613.2001.iep0082-0193-x PMC Full Text View journal details
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