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PMID: 11483525 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Non-canonical mechanism for translational control in bacteria: synthesis of ribosomal protein S1.

The EMBO journal ·Vol. 20 ·No. 15 ·2001-08-01 ·Pages 4222-32

Boni IV, Artamonova VS, Tzareva NV, Dreyfus M

Abstract

Translation initiation region (TIR) of the rpsA mRNA encoding ribosomal protein S1 is one of the most efficient in Escherichia coli despite the absence of a canonical Shine-Dalgarno-element. Its high efficiency is under strong negative autogenous control, a puzzling phenomenon as S1 has no strict sequence specificity. To define sequence and structural elements responsible for translational efficiency and autoregulation of the rpsA mRNA, a series of rpsA'-'lacZ chromosomal fusions bearing various mutations in the rpsA TIR was created and tested for beta-galactosidase activity in the absence and presence of excess S1. These in vivo results, as well as data obtained by in vitro techniques and phylogenetic comparison, allow us to propose a model for the structural and functional organization of the rpsA TIR specific for proteobacteria related to E.coli. According to the model, the high efficiency of translation initiation is provided by a specific fold of the rpsA leader forming a non-contiguous ribosome entry site, which is destroyed upon binding of free S1 when it acts as an autogenous repressor.

MeSH Terms
5' Untranslated Regions Bacterial Proteins/biosynthesis,genetics Base Sequence Binding Sites Escherichia coli/genetics Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Conformation Peptide Chain Initiation, Translational Phylogeny Protein Biosynthesis Proteobacteria/genetics RNA, Bacterial/chemistry Ribosomal Proteins/biosynthesis,genetics
Chemicals
5' Untranslated Regions Bacterial Proteins RNA, Bacterial Ribosomal Proteins ribosomal protein S1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Boni I V
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117871 Moscow, Russia. irina@humgen.siobc.ras.ru
Artamonova V S
Tzareva N V
Dreyfus M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.15.4222
Email
irina@humgen.siobc.ras.ru
Published
2001-08-01
Pages
4222-32
Language
English
Region
England
NLM ID
8208664
PMCID
PMC149162
Subset
IM
Databases
GENBANK
AE003852, AP001118, L42023, L43549
External Links
PubMed source DOI: 10.1093/emboj/20.15.4222 PMC Full Text View journal details
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