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PMID: 11470821 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Golgi clusters and vesicles mediate mitotic inheritance independently of the endoplasmic reticulum.

The Journal of cell biology ·Vol. 154 ·No. 2 ·2001-07-23 ·Pages 317-30

Jokitalo E, Cabrera-Poch N, Warren G, Shima DT

Abstract

We have examined the fate of Golgi membranes during mitotic inheritance in animal cells using four-dimensional fluorescence microscopy, serial section reconstruction of electron micrographs, and peroxidase cytochemistry to track the fate of a Golgi enzyme fused to horseradish peroxidase. All three approaches show that partitioning of Golgi membranes is mediated by Golgi clusters that persist throughout mitosis, together with shed vesicles that are often found associated with spindle microtubules. We have been unable to find evidence that Golgi membranes fuse during the later phases of mitosis with the endoplasmic reticulum (ER) as a strategy for Golgi partitioning (Zaal, K.J., C.L. Smith, R.S. Polishchuk, N. Altan, N.B. Cole, J. Ellenberg, K. Hirschberg, J.F. Presley, T.H. Roberts, E. Siggia, et al. 1999. Cell. 99:589-601) and suggest that these results, in part, are the consequence of slow or abortive folding of GFP-Golgi chimeras in the ER. Furthermore, we show that accurate partitioning is accomplished early in mitosis, by a process of cytoplasmic redistribution of Golgi fragments and vesicles yielding a balance of Golgi membranes on either side of the metaphase plate before cell division.

MeSH Terms
Animals Cell Line Cricetinae Cytoplasmic Vesicles/metabolism,ultrastructure Endoplasmic Reticulum/metabolism,ultrastructure Golgi Apparatus/metabolism,ultrastructure Horseradish Peroxidase/genetics Humans Intracellular Membranes/metabolism Metaphase/physiology Microscopy, Fluorescence/methods Microtubules/metabolism Mitosis/physiology N-Acetylglucosaminyltransferases/genetics N-Acetyllactosamine Synthase/genetics Rats Recombinant Fusion Proteins/genetics,metabolism Spindle Apparatus/metabolism Telophase/physiology Transfection
Chemicals
Recombinant Fusion Proteins Horseradish Peroxidase N-Acetylglucosaminyltransferases alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I N-Acetyllactosamine Synthase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jokitalo E
Institute of Biotechnology, Electron Microscopy Unit, University of Helsinki, 00014 Helsinki, Finland.
Cabrera-Poch N
Warren G
Shima D T
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200104073
Published
2001-07-23
Pages
317-30
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2150754
Subset
IM
Grants
NIGMS NIH HHS · R01 GM060478 · United States
NIGMS NIH HHS · GM60478-01 · United States
External Links
PubMed source DOI: 10.1083/jcb.200104073 PMC Full Text View journal details
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