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PMID: 11459973 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Managing DNA polymerases: coordinating DNA replication, DNA repair, and DNA recombination.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 15 ·2001-07-17 ·Pages 8342-9

Sutton MD, Walker GC

Abstract

Two important and timely questions with respect to DNA replication, DNA recombination, and DNA repair are: (i) what controls which DNA polymerase gains access to a particular primer-terminus, and (ii) what determines whether a DNA polymerase hands off its DNA substrate to either a different DNA polymerase or to a different protein(s) for the completion of the specific biological process? These questions have taken on added importance in light of the fact that the number of known template-dependent DNA polymerases in both eukaryotes and in prokaryotes has grown tremendously in the past two years. Most notably, the current list now includes a completely new family of enzymes that are capable of replicating imperfect DNA templates. This UmuC-DinB-Rad30-Rev1 superfamily of DNA polymerases has members in all three kingdoms of life. Members of this family have recently received a great deal of attention due to the roles they play in translesion DNA synthesis (TLS), the potentially mutagenic replication over DNA lesions that act as potent blocks to continued replication catalyzed by replicative DNA polymerases. Here, we have attempted to summarize our current understanding of the regulation of action of DNA polymerases with respect to their roles in DNA replication, TLS, DNA repair, DNA recombination, and cell cycle progression. In particular, we discuss these issues in the context of the Gram-negative bacterium, Escherichia coli, that contains a DNA polymerase (Pol V) known to participate in most, if not all, of these processes.

MeSH Terms
Animals Bacterial Proteins/metabolism DNA Damage DNA Polymerase III/metabolism DNA Primers DNA Repair DNA Replication DNA-Directed DNA Polymerase/metabolism Escherichia coli/enzymology Escherichia coli Proteins Eukaryotic Cells Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Prokaryotic Cells Rec A Recombinases/metabolism Recombination, Genetic Signal Transduction Templates, Genetic
Chemicals
Bacterial Proteins DNA Primers Escherichia coli Proteins UmuC protein, E coli Rec A Recombinases DNA Polymerase III DNA polymerase V, E coli DNA-Directed DNA Polymerase UmuD protein, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sutton M D
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Walker G C
References (117)
117 references, click to expand
  1. Proteins required for ultraviolet light and chemical mutagenesis. Identification of the products of the umuC locus of Escherichia coli.
    J Mol Biol. 1983 Feb 25;164(2):175-92 PMID: 6302271
  2. Xeroderma pigmentosum variant (XP-V) correcting protein from HeLa cells has a thymine dimer bypass DNA polymerase activity.
    EMBO J. 1999 Jun 15;18(12):3491-501 PMID: 10369688
  3. The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase eta.
    Nature. 1999 Jun 17;399(6737):700-4 PMID: 10385124
  4. DNA recombination: the replication connection.
    Trends Biochem Sci. 1999 Jul;24(7):271-5 PMID: 10390616
  5. Cloning and chromosomal mapping of the human DNA polymerase theta (POLQ), the eighth human DNA polymerase.
    Genomics. 1999 Jul 1;59(1):90-6 PMID: 10395804
  6. hRAD30 mutations in the variant form of xeroderma pigmentosum.
    Science. 1999 Jul 9;285(5425):263-5 PMID: 10398605
  7. UmuD'(2)C is an error-prone DNA polymerase, Escherichia coli pol V.
    Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8919-24 PMID: 10430871
  8. Differential assembly of Cdc45p and DNA polymerases at early and late origins of DNA replication.
    Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9130-5 PMID: 10430907
  9. A model for a umuDC-dependent prokaryotic DNA damage checkpoint.
    Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9218-23 PMID: 10430923
  10. Efficient processing of DNA ends during yeast nonhomologous end joining. Evidence for a DNA polymerase beta (Pol4)-dependent pathway.
    J Biol Chem. 1999 Aug 13;274(33):23599-609 PMID: 10438542
  11. Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta.
    Genomics. 1999 Aug 15;60(1):20-30 PMID: 10458907
  12. Novel DNA polymerases offer clues to the molecular basis of mutagenesis.
    Cell. 1999 Aug 20;98(4):413-6 PMID: 10481906
  13. A plethora of lesion-replicating DNA polymerases.
    Genes Dev. 1999 Sep 1;13(17):2191-5 PMID: 10485842
  14. The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis.
    Mol Cell. 1999 Aug;4(2):281-6 PMID: 10488344
  15. Human and mouse homologs of Escherichia coli DinB (DNA polymerase IV), members of the UmuC/DinB superfamily.
    Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11922-7 PMID: 10518552
  16. Visualization of two binding sites for the Escherichia coli UmuD'(2)C complex (DNA pol V) on RecA-ssDNA filaments.
    J Mol Biol. 2000 Mar 31;297(3):585-97 PMID: 10731413
  17. DNA polymerase mu (Pol mu), homologous to TdT, could act as a DNA mutator in eukaryotic cells.
    EMBO J. 2000 Apr 3;19(7):1731-42 PMID: 10747040
  18. Replication and recombination intersect.
    Curr Opin Genet Dev. 2000 Apr;10(2):151-6 PMID: 10753777
  19. PriA-directed replication fork restart in Escherichia coli.
    Trends Biochem Sci. 2000 Apr;25(4):185-9 PMID: 10754552
  20. Coping with replication 'train wrecks' in Escherichia coli using Pol V, Pol II and RecA proteins.
    Trends Biochem Sci. 2000 Apr;25(4):189-95 PMID: 10754553
  21. Dynamics of DNA replication factories in living cells.
    J Cell Biol. 2000 Apr 17;149(2):271-80 PMID: 10769021
  22. Roles of E. coli DNA polymerases IV and V in lesion-targeted and untargeted SOS mutagenesis.
    Nature. 2000 Apr 27;404(6781):1014-8 PMID: 10801133
  23. The many faces of DNA polymerases: strategies for mutagenesis and for mutational avoidance.
    Proc Natl Acad Sci U S A. 2000 May 23;97(11):5681-3 PMID: 10811923
  24. Error-prone bypass of certain DNA lesions by the human DNA polymerase kappa.
    Genes Dev. 2000 Jul 1;14(13):1589-94 PMID: 10887153
  25. poliota, a remarkably error-prone human DNA polymerase.
    Genes Dev. 2000 Jul 1;14(13):1642-50 PMID: 10887158
  26. Replication of UV-damaged DNA: new insights into links between DNA polymerases, mutagenesis and human disease.
    Gene. 2000 Jul 25;253(1):1-12 PMID: 10925197
  27. Pol kappa: A DNA polymerase required for sister chromatid cohesion.
    Science. 2000 Aug 4;289(5480):774-9 PMID: 10926539
  28. Structural design of a eukaryotic DNA repair polymerase: DNA polymerase beta.
    Mutat Res. 2000 Aug 30;460(3-4):231-44 PMID: 10946231
  29. DNA polymerase lambda (Pol lambda), a novel eukaryotic DNA polymerase with a potential role in meiosis.
    J Mol Biol. 2000 Aug 25;301(4):851-67 PMID: 10966791
  30. Eukaryotic polymerases iota and zeta act sequentially to bypass DNA lesions.
    Nature. 2000 Aug 31;406(6799):1015-9 PMID: 10984059
  31. Two novel human and mouse DNA polymerases of the polX family.
    Nucleic Acids Res. 2000 Sep 15;28(18):3684-93 PMID: 10982892
  32. DNA polymerases and SOS mutagenesis: can one reconcile the biochemical and genetic data?
    Bioessays. 2000 Oct;22(10):933-7 PMID: 10984719
  33. A coordinated interplay: proteins with multiple functions in DNA replication, DNA repair, cell cycle/checkpoint control, and transcription.
    Prog Nucleic Acid Res Mol Biol. 2001;65:261-98 PMID: 11008490
  34. Xeroderma pigmentosum and related disorders: defects in DNA repair and transcription.
    Adv Genet. 2001;43:71-102 PMID: 11037299
  35. Subunit-specific degradation of the UmuD/D' heterodimer by the ClpXP protease: the role of trans recognition in UmuD' stability.
    EMBO J. 2000 Oct 2;19(19):5251-8 PMID: 11013227
  36. All three SOS-inducible DNA polymerases (Pol II, Pol IV and Pol V) are involved in induced mutagenesis.
    EMBO J. 2000 Nov 15;19(22):6259-65 PMID: 11080171
  37. Fidelity and processivity of DNA synthesis by DNA polymerase kappa, the product of the human DINB1 gene.
    J Biol Chem. 2000 Dec 15;275(50):39678-84 PMID: 11006276
  38. Purification and characterization of pol kappa, a DNA polymerase encoded by the human DINB1 gene.
    J Biol Chem. 2001 Jan 5;276(1):92-8 PMID: 11024016
  39. The SOS response: recent insights into umuDC-dependent mutagenesis and DNA damage tolerance.
    Annu Rev Genet. 2000;34:479-497 PMID: 11092836
  40. The C-terminal domain of dnaQ contains the polymerase binding site.
    J Bacteriol. 1999 May;181(9):2963-5 PMID: 10217794
  41. The organization of replication and transcription.
    Science. 1999 Jun 11;284(5421):1790-5 PMID: 10364545
  42. Bridging the gap: a family of novel DNA polymerases that replicate faulty DNA.
    Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12224-6 PMID: 10535901
  43. The Escherichia coli SOS mutagenesis proteins UmuD and UmuD' interact physically with the replicative DNA polymerase.
    Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12373-8 PMID: 10535929
  44. The human REV1 gene codes for a DNA template-dependent dCMP transferase.
    Nucleic Acids Res. 1999 Nov 15;27(22):4468-75 PMID: 10536157
  45. The mutagenesis protein UmuC is a DNA polymerase activated by UmuD', RecA, and SSB and is specialized for translesion replication.
    J Biol Chem. 1999 Nov 5;274(45):31763-6 PMID: 10542196
  46. Multiple competition reactions for RPA order the assembly of the DNA polymerase delta holoenzyme.
    EMBO J. 1999 Nov 1;18(21):6189-99 PMID: 10545128
  47. Replisome assembly at oriC, the replication origin of E. coli, reveals an explanation for initiation sites outside an origin.
    Mol Cell. 1999 Oct;4(4):541-53 PMID: 10549286
  48. Replication of damaged DNA: molecular defect in xeroderma pigmentosum variant cells.
    Mutat Res. 1999 Oct 22;435(2):111-9 PMID: 10556591
  49. PCNA binding proteins.
    Front Biosci. 1999 Dec 1;4:D849-58 PMID: 10577396
  50. Two functional domains of the epsilon subunit of DNA polymerase III.
    Biochemistry. 1999 Nov 30;38(48):16001-9 PMID: 10625468
  51. A role for the umuDC gene products of Escherichia coli in increasing resistance to DNA damage in stationary phase by inhibiting the transition to exponential growth.
    J Bacteriol. 2000 Feb;182(4):1127-35 PMID: 10648540
  52. Mammalian DNA mismatch repair.
    Annu Rev Genet. 1999;33:533-64 PMID: 10690417
  53. The importance of repairing stalled replication forks.
    Nature. 2000 Mar 2;404(6773):37-41 PMID: 10716434
  54. Autodigestion of lexA and phage lambda repressors.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1375-9 PMID: 6231641
  55. Cold sensitivity induced by overproduction of UmuDC in Escherichia coli.
    J Bacteriol. 1985 Apr;162(1):155-61 PMID: 2984171
  56. Mechanism of transient inhibition of DNA synthesis in ultraviolet-irradiated E. coli: inhibition is independent of recA whilst recovery requires RecA protein itself and an additional, inducible SOS function.
    Mol Gen Genet. 1985;199(1):133-40 PMID: 3889546
  57. umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4331-5 PMID: 2989816
  58. Recovery from ultraviolet light-induced inhibition of DNA synthesis requires umuDC gene products in recA718 mutant strains but not in recA+ strains of Escherichia coli.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6805-9 PMID: 3309946
  59. RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis.
    Proc Natl Acad Sci U S A. 1988 Mar;85(6):1806-10 PMID: 3126496
  60. UmuD mutagenesis protein of Escherichia coli: overproduction, purification, and cleavage by RecA.
    Proc Natl Acad Sci U S A. 1988 Mar;85(6):1811-5 PMID: 3279417
  61. RecA-mediated cleavage activates UmuD for mutagenesis: mechanistic relationship between transcriptional derepression and posttranslational activation.
    Proc Natl Acad Sci U S A. 1988 Mar;85(6):1816-20 PMID: 3279418
  62. The REV1 gene of Saccharomyces cerevisiae: isolation, sequence, and functional analysis.
    J Bacteriol. 1989 Jan;171(1):230-7 PMID: 2492497
  63. UmuC mutagenesis protein of Escherichia coli: purification and interaction with UmuD and UmuD'.
    Proc Natl Acad Sci U S A. 1989 Oct;86(19):7301-5 PMID: 2552436
  64. Nature of the SOS-inducing signal in Escherichia coli. The involvement of DNA replication.
    J Mol Biol. 1990 Mar 5;212(1):79-96 PMID: 2108251
  65. Dominant negative umuD mutations decreasing RecA-mediated cleavage suggest roles for intact UmuD in modulation of SOS mutagenesis.
    Proc Natl Acad Sci U S A. 1990 Sep;87(18):7190-4 PMID: 2205854
  66. Replication factors required for SV40 DNA replication in vitro. II. Switching of DNA polymerase alpha and delta during initiation of leading and lagging strand synthesis.
    J Biol Chem. 1991 Jan 25;266(3):1961-8 PMID: 1671046
  67. Fidelity mechanisms in DNA replication.
    Annu Rev Biochem. 1991;60:477-511 PMID: 1883202
  68. A RecA protein mutant deficient in its interaction with the UmuDC complex.
    Biochimie. 1991 Apr;73(4):479-84 PMID: 1911948
  69. Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen.
    EMBO J. 1992 Feb;11(2):769-76 PMID: 1311258
  70. Overproduction of the beta subunit of DNA polymerase III holoenzyme reduces UV mutagenesis in Escherichia coli.
    J Bacteriol. 1992 Apr;174(8):2517-24 PMID: 1556072
  71. Targeting of the UmuD, UmuD', and MucA' mutagenesis proteins to DNA by RecA protein.
    Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8169-73 PMID: 8367479
  72. The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interaction with PCNA.
    Nature. 1994 Jun 16;369(6481):574-8 PMID: 7911228
  73. The appearance of the UmuD'C protein complex in Escherichia coli switches repair from homologous recombination to SOS mutagenesis.
    Mol Microbiol. 1993 Dec;10(5):963-71 PMID: 7934872
  74. DNA polymerase epsilon links the DNA replication machinery to the S phase checkpoint.
    Cell. 1995 Jan 13;80(1):29-39 PMID: 7813016
  75. DNA polymerase III holoenzyme: structure and function of a chromosomal replicating machine.
    Annu Rev Biochem. 1995;64:171-200 PMID: 7574479
  76. Purification of a soluble UmuD'C complex from Escherichia coli. Cooperative binding of UmuD'C to single-stranded DNA.
    J Biol Chem. 1996 May 3;271(18):10767-74 PMID: 8631887
  77. Thymine-thymine dimer bypass by yeast DNA polymerase zeta.
    Science. 1996 Jun 14;272(5268):1646-9 PMID: 8658138
  78. In vivo stability of the Umu mutagenesis proteins: a major role for RecA.
    J Bacteriol. 1996 Jun;178(12):3550-6 PMID: 8655553
  79. The genetic requirements for UmuDC-mediated cold sensitivity are distinct from those for SOS mutagenesis.
    J Bacteriol. 1996 Aug;178(15):4400-11 PMID: 8755866
  80. A model for SOS-lesion-targeted mutations in Escherichia coli.
    Nature. 2001 Jan 18;409(6818):366-70 PMID: 11201748
  81. umuDC-mediated cold sensitivity is a manifestation of functions of the UmuD(2)C complex involved in a DNA damage checkpoint control.
    J Bacteriol. 2001 Feb;183(4):1215-24 PMID: 11157933
  82. Deoxycytidyl transferase activity of yeast REV1 protein.
    Nature. 1996 Aug 22;382(6593):729-31 PMID: 8751446
  83. Regulation of SOS mutagenesis by proteolysis.
    Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10291-6 PMID: 8816793
  84. Cell cycle checkpoints: preventing an identity crisis.
    Science. 1996 Dec 6;274(5293):1664-72 PMID: 8939848
  85. A role for DNA primase in coupling DNA replication to DNA damage response.
    EMBO J. 1997 Feb 3;16(3):639-50 PMID: 9034345
  86. Quantitation of the inhibition of Hfr x F- recombination by the mutagenesis complex UmuD'C.
    J Mol Biol. 1997 Jul 11;270(2):201-11 PMID: 9236122
  87. The mutagenesis protein MucB interacts with single strand DNA binding protein and induces a major conformational change in its complex with single-stranded DNA.
    J Biol Chem. 1998 Mar 6;273(10):5520-7 PMID: 9488676
  88. Devoted to the lagging strand-the subunit of DNA polymerase III holoenzyme contacts SSB to promote processive elongation and sliding clamp assembly.
    EMBO J. 1998 Apr 15;17(8):2436-49 PMID: 9545254
  89. Mutagenesis and more: umuDC and the Escherichia coli SOS response.
    Genetics. 1998 Apr;148(4):1599-610 PMID: 9560379
  90. Inhibition of Escherichia coli RecA coprotease activities by DinI.
    EMBO J. 1998 Jun 1;17(11):3207-16 PMID: 9606202
  91. DNA ligase I is recruited to sites of DNA replication by an interaction with proliferating cell nuclear antigen: identification of a common targeting mechanism for the assembly of replication factories.
    EMBO J. 1998 Jul 1;17(13):3786-95 PMID: 9649448
  92. Mammalian base excision repair and DNA polymerase beta.
    Mutat Res. 1998 Jun;407(3):203-15 PMID: 9653447
  93. Biochemical basis of SOS-induced mutagenesis in Escherichia coli: reconstitution of in vitro lesion bypass dependent on the UmuD'2C mutagenic complex and RecA protein.
    Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9755-60 PMID: 9707548
  94. The chi psi subunits of DNA polymerase III holoenzyme bind to single-stranded DNA-binding protein (SSB) and facilitate replication of an SSB-coated template.
    J Biol Chem. 1998 Sep 4;273(36):23476-84 PMID: 9722585
  95. DNA damage and checkpoint pathways: molecular anatomy and interactions with repair.
    Cell. 1998 Sep 4;94(5):555-8 PMID: 9741620
  96. The DNA replication fork in eukaryotic cells.
    Annu Rev Biochem. 1998;67:721-51 PMID: 9759502
  97. Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity.
    Cell. 1998 Oct 2;95(1):135-46 PMID: 9778254
  98. Eukaryotic DNA polymerases in DNA replication and DNA repair.
    Chromosoma. 1998 Sep;107(4):218-27 PMID: 9745046
  99. Localization of bacterial DNA polymerase: evidence for a factory model of replication.
    Science. 1998 Nov 20;282(5393):1516-9 PMID: 9822387
  100. Modulation of RecA nucleoprotein function by the mutagenic UmuD'C protein complex.
    J Biol Chem. 1998 Dec 4;273(49):32384-7 PMID: 9829966
  101. Lon-mediated proteolysis of the Escherichia coli UmuD mutagenesis protein: in vitro degradation and identification of residues required for proteolysis.
    Genes Dev. 1998 Dec 15;12(24):3889-99 PMID: 9869642
  102. Efficient bypass of a thymine-thymine dimer by yeast DNA polymerase, Poleta.
    Science. 1999 Feb 12;283(5404):1001-4 PMID: 9974380
  103. Trading places on DNA--a three-point switch underlies primer handoff from primase to the replicative DNA polymerase.
    Cell. 1999 Jan 8;96(1):153-63 PMID: 9989506
  104. Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases.
    Cell. 1999 Feb 5;96(3):415-24 PMID: 10025407
  105. Eukaryotic DNA mismatch repair.
    Curr Opin Genet Dev. 1999 Feb;9(1):89-96 PMID: 10072354
  106. Specificity of DNA lesion bypass by the yeast DNA polymerase eta.
    J Biol Chem. 2000 Mar 17;275(11):8233-9 PMID: 10713149
  107. Movement of replicating DNA through a stationary replisome.
    Mol Cell. 2000 Dec;6(6):1321-30 PMID: 11163206
  108. umuDC-dnaQ Interaction and its implications for cell cycle regulation and SOS mutagenesis in Escherichia coli.
    J Bacteriol. 2001 Feb;183(3):1085-9 PMID: 11208808
  109. Genetic interactions between the Escherichia coli umuDC gene products and the beta processivity clamp of the replicative DNA polymerase.
    J Bacteriol. 2001 May;183(9):2897-909 PMID: 11292811
  110. Bryn Bridges and mutagenesis: exploring the intellectual space.
    Mutat Res. 2001 Feb 25;485(1):69-81 PMID: 11341995
  111. Interaction of the beta sliding clamp with MutS, ligase, and DNA polymerase I.
    Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8376-80 PMID: 11459978
  112. Pathways of ultraviolet mutability in Saccharomyces cerevisiae. I. Some properties of double mutants involving uvs9 and rev.
    Mutat Res. 1971 Dec;13(4):311-7 PMID: 4947374
  113. Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light.
    Mol Gen Genet. 1977 Nov 14;156(2):121-31 PMID: 340898
  114. Uvm mutants of Escherichia coli K12 deficient in UV mutagenesis. I. Isolation of uvm mutants and their phenotypical characterization in DNA repair and mutagenesis.
    Mol Gen Genet. 1978 Sep 20;165(1):87-93 PMID: 362169
  115. DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2819-23 PMID: 6771759
  116. Cleavage of the Escherichia coli lexA protein by the recA protease.
    Proc Natl Acad Sci U S A. 1980 Jun;77(6):3225-9 PMID: 6447873
  117. Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5749-53 PMID: 7029544
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.111036998
Published
2001-07-17
Pages
8342-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC37441
Subset
IM
Grants
NCI NIH HHS · F32 CA079161 · United States
NCI NIH HHS · R01 CA021615 · United States
NCI NIH HHS · 5 F32 CA79161-03 · United States
NCI NIH HHS · CA21615 · United States
External Links
PubMed source DOI: 10.1073/pnas.111036998 PMC Full Text View journal details
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