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PMID: 11454057 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Systematic characterization of human CD8+ T cells with natural killer cell markers in comparison with natural killer cells and normal CD8+ T cells.

Immunology ·Vol. 103 ·No. 3 ·2001-07-00 ·Pages 281-90

Ohkawa T, Seki S, Dobashi H, Koike Y, Habu Y, Ami K, Hiraide H, Sekine I

Abstract

We investigated the function of CD56+ CD8+ T cells (CD56+ T cells) and CD56- CD57+ CD8+ T cells (CD57+ T cells; natural killer (NK)-type T cells) and compared them with those of normal CD56- CD57- CD8+ T cells (CD8+ T cells) and CD56+ NK cells from healthy volunteers. After the stimulation with immobilized anti-CD3 antibodies, both NK-type T cells produced much larger amounts of interferon-gamma (IFN-gamma) than CD8+ T cells. Both NK-type T cells also acquired a more potent cytotoxicity against NK-sensitive K562 cells than CD8+ T cells while only CD56+ T cells showed a potent cytotoxicity against NK-resistant Raji cells. After the stimulation with a combination of interleukin (IL)-2, IL-12 and IL-15, the IFN-gamma amounts produced were NK cells > or = CD56+ T cells > or = CD57+ T cells > CD8+ T cells. The cytotoxicities against K562 cells were NK cells > CD56+ T cells > or = CD57+ T cells > CD8+ T cells while cytotoxicities against Raji cells were CD56+ T cells > CD57+ T cells > or = CD8+ T cells > or = NK cells. However, the CD3-stimulated proliferation of both NK-type T cells was smaller than that of CD8+ T cells partly because NK-type T cells were susceptible to apoptosis. In addition to NK cells, NK-type T cells but not CD8+ T cells stimulated with cytokines, expressed cytoplasmic perforin and granzyme B. Furthermore, CD3-stimulated IFN-gamma production from peripheral blood mononuclear cells (PBMC) correlated with the proportions of CD57+ T cells in PBMC from donors. Our findings suggest that NK-type T cells play an important role in the T helper 1 responses and the immunological changes associated with ageing.

MeSH Terms
Adult Apoptosis/immunology CD3 Complex/immunology CD56 Antigen/analysis CD57 Antigens/analysis CD8-Positive T-Lymphocytes/immunology Cell Culture Techniques Cell Division/immunology Child Cytotoxicity, Immunologic Granzymes Humans Immunophenotyping Interferon-gamma/biosynthesis K562 Cells/immunology Killer Cells, Natural/immunology Lymphocyte Activation/immunology Membrane Glycoproteins/metabolism Perforin Pore Forming Cytotoxic Proteins Receptors, Antigen, T-Cell, alpha-beta/analysis Serine Endopeptidases/metabolism T-Lymphocyte Subsets/immunology Tumor Cells, Cultured
Chemicals
CD3 Complex CD56 Antigen CD57 Antigens Membrane Glycoproteins Pore Forming Cytotoxic Proteins Receptors, Antigen, T-Cell, alpha-beta Perforin Interferon-gamma GZMB protein, human Granzymes Serine Endopeptidases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ohkawa T
Department of Pediatrics, National Defense Medical College, Tokorozawa, Japan.
Seki S
Dobashi H
Koike Y
Habu Y
Ami K
Hiraide H
Sekine I
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
DOI
10.1046/j.1365-2567.2001.01248.x
Published
2001-07-00
Pages
281-90
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1783250
Subset
IM
External Links
PubMed source DOI: 10.1046/j.1365-2567.2001.01248.x PMC Full Text View journal details
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