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PMID: 11438678 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Drosophila Med6 is required for elevated expression of a large but distinct set of developmentally regulated genes.

Molecular and cellular biology ·Vol. 21 ·No. 15 ·2001-08-00 ·Pages 5242-55

Gim BS, Park JM, Yoon JH, Kang C, Kim YJ

Abstract

Mediator is the evolutionarily conserved coactivator required for the integration and recruitment of diverse regulatory signals to basal transcription machinery. To elucidate the functions of metazoan Mediator, we isolated Drosophila melanogaster Med6 mutants. dMed6 is essential for viability and/or proliferation of most cells. dMed6 mutants failed to pupate and died in the third larval instar with severe proliferation defects in imaginal discs and other larval mitotic cells. cDNA microarray, quantitative reverse transcription-PCR, and in situ expression analyses of developmentally regulated genes in dMed6 mutants showed that transcriptional activation of many, but not all, genes was affected. Among the genes found to be affected were some that play a role in cell proliferation and metabolism. Therefore, dMed6 is required in most cells for transcriptional regulation of many genes important for diverse aspects of Drosophila development.

MeSH Terms
Alleles Animals Blotting, Northern Cell Division Cell Nucleus Cell Survival Cloning, Molecular DNA Nucleotidyltransferases/metabolism DNA, Complementary/metabolism Drosophila Drosophila Proteins Fungal Proteins/metabolism,physiology Green Fluorescent Proteins Homozygote Lac Operon Luminescent Proteins/metabolism Mediator Complex Mutation Oligonucleotide Array Sequence Analysis Phenotype Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational Promoter Regions, Genetic Recombinant Fusion Proteins/metabolism Reverse Transcriptase Polymerase Chain Reaction Saccharomyces cerevisiae Proteins Time Factors Tissue Distribution Trans-Activators Transcription, Genetic Transcriptional Activation
Chemicals
DNA, Complementary Drosophila Proteins Fungal Proteins Luminescent Proteins Med6 protein, Drosophila Mediator Complex Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Trans-Activators Green Fluorescent Proteins DNA Nucleotidyltransferases FLP recombinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gim B S
Department of Biochemistry, National Creative Research Initiative Center for Genome Regulation, Yonsei University, Seoul 120-749, Korea.
Park J M
Yoon J H
Kang C
Kim Y J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.21.15.5242-5255.2001
Published
2001-08-00
Pages
5242-55
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC87248
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.21.15.5242-5255.2001 PMC Full Text View journal details
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