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PMID: 11414611 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Molecular identification and characterization of the Arabidopsis AtADF1, AtADFS and AtADF6 genes.

Plant molecular biology ·Vol. 45 ·No. 5 ·2001-03-00 ·Pages 517-27

Dong CH, Kost B, Xia G, Chua NH

Abstract

Actin depolymerizing factor (ADF) is a key regulator of the organization of the actin cytoskeleton during various cellular activities. We found that ADF genes in Arabidopsis form a large family consisting of at least nine members, four of which were cloned and sequenced in this study. Comparison of genomic and cDNA sequences showed that the AtADF1, AtADF5, and AtADF6 genes all contain two introns at conserved positions. Analysis of transgenic Arabidopsis plants carrying promoter-GUS fusion constructs revealed that AtADF1 and AtADF6 are expressed in the vascular tissues of all organs, whereas expression of AtADF5 is restricted to the root tip meristem. GFP-AtADFI, GFP-AtADF5, and GFP-AtADF6 fusion proteins were found to bind to actin filaments in vivo, and to reorganize the actin cytoskeleton when transiently expressed in plant cells.

MeSH Terms
Actin Depolymerizing Factors Actins/metabolism Amino Acid Sequence Arabidopsis/genetics,metabolism Blotting, Northern Cloning, Molecular Cytoskeletal Proteins/genetics,metabolism Destrin Expressed Sequence Tags Glucuronidase/genetics,metabolism Green Fluorescent Proteins Introns Luminescent Proteins/genetics,metabolism Microfilament Proteins/genetics,metabolism Microscopy, Fluorescence Molecular Sequence Data Plant Structures/anatomy & histology,metabolism RNA, Messenger/analysis Recombinant Fusion Proteins/genetics,metabolism Sequence Alignment
Chemicals
Actin Depolymerizing Factors Actins Cytoskeletal Proteins Destrin Luminescent Proteins Microfilament Proteins RNA, Messenger Recombinant Fusion Proteins Green Fluorescent Proteins Glucuronidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dong C H
Laboratory of Plant Cell Biology, Institute of Molecular Agrobiology, National University of Singapore, Singapore.
Kost B
Xia G
Chua N H
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43 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1010687911374
Published
2001-03-00
Pages
517-27
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
AF102173, AF102825, AF183576
External Links
PubMed source DOI: 10.1023/a:1010687911374 View journal details
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