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PMID: 11404480 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Evolutionary EST analysis identifies rapidly evolving male reproductive proteins in Drosophila.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 13 ·2001-06-19 ·Pages 7375-9

Swanson WJ, Clark AG, Waldrip-Dail HM, Wolfner MF, Aquadro CF

Abstract

Sequence comparisons of genomes or expressed sequence tags (ESTs) from related organisms provide insight into functional conservation and diversification. We compare the sequences of ESTs from the male accessory gland of Drosophila simulans to their orthologs in its close relative Drosophila melanogaster, and demonstrate rapid divergence of many of these reproductive genes. Nineteen ( approximately 11%) of 176 independent genes identified in the EST screen contain protein-coding regions with an excess of nonsynonymous over synonymous changes, suggesting that their divergence has been accelerated by positive Darwinian selection. Genes that encode putative accessory gland-specific seminal fluid proteins had a significantly elevated level of nonsynonymous substitution relative to nonaccessory gland-specific genes. With the 57 new accessory gland genes reported here, we predict that approximately 90% of the male accessory gland genes have been identified. The evolutionary EST approach applied here to identify putative targets of adaptive evolution is readily applicable to other tissues and organisms.

MeSH Terms
Analysis of Variance Animals Drosophila/classification,genetics Drosophila melanogaster/genetics Evolution, Molecular Expressed Sequence Tags Genitalia, Male/physiology Male Molecular Sequence Data Proteins/genetics
Chemicals
Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Swanson W J
Department of Molecular Biology and Genetics, Biotechnology Building, Cornell University, Ithaca, NY 14853-2703, USA. wjs18@cornell.edu
Clark A G
Waldrip-Dail H M
Wolfner M F
Aquadro C F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.131568198
Email
wjs18@cornell.edu
Published
2001-06-19
Epub
2001-00-12
Pages
7375-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC34676
Subset
IM
Grants
NIGMS NIH HHS · R01 GM036431 · United States
NICHD NIH HHS · R01 HD038921 · United States
NIGMS NIH HHS · GM36431 · United States
NICHD NIH HHS · HD38921 · United States
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PubMed source DOI: 10.1073/pnas.131568198 PMC Full Text View journal details
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