Cross-bridge conformation as revealed by x-ray diffraction studies on insect flight muscles with ATP analogues.

Biophysical journal ·Vol. 15 ·No. 7 ·1975-07-00 ·Pages 687-705

Goody RS, Holmes KC, Mannherz HG, Leigh JB, Rosenbaum G

Abstract

The effects of three ATP analogues, alpha,beta-methylene-ATP [ATP(alpha,beta-CH1)], adenosine 5'-0-(3-thiotrophosphate) [ATP(gamma-S)], and beta,gamma-amino-ATP [ATP(beta,gamma-NH)] at various concentrations and temperatures on the X-ray fiber diagrams of glycerinated flight muscles from a water bug (Lethocerus maximus) have been investigated. It is shown that the "relaxed" state can be obtained with all three analogues at high concentrations, the result being particularly clear with ATP(gamma-S). It is inferred that the binding of an ATP-like molecule suffices to produce the relaxed state. At low concentrations ATP(beta,gamma-NH) produces state intermediate between rigor and relaxed which is not simply a mixture of the two. The possible nature of the intermediate is discussed.

MeSH Terms

Actins Actomyosin Adenosine Triphosphate/analogs & derivatives Animals Binding Sites Flight, Animal Glycerol Insecta Kinetics Mathematics Muscles/ultrastructure Myosins Protein Binding Protein Conformation Temperature X-Ray Diffraction

Chemicals

Actins Adenosine Triphosphate Actomyosin Myosins Glycerol

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Goody R S

Holmes K C

Mannherz H G

Leigh J B

Rosenbaum G

References (28)

28 references, click to expand

Muscle structure and theories of contraction.
Prog Biophys Biophys Chem. 1957;7:255-318 PMID: 13485191
The double array of filaments in cross-striated muscle.
J Biophys Biochem Cytol. 1957 Sep 25;3(5):631-48 PMID: 13475381
Changes in the cross-striations of muscle during contraction and stretch and their structural interpretation.
Nature. 1954 May 22;173(4412):973-6 PMID: 13165698
Structural changes in muscle during contraction; interference microscopy of living muscle fibres.
Nature. 1954 May 22;173(4412):971-3 PMID: 13165697
Structural basis of the cross-striations in muscle.
Nature. 1953 Sep 19;172(4377):530-2 PMID: 13099257
Structure of insect fibrillar flight muscle in the presence and absence of ATP.
J Mol Biol. 1972 Sep 14;70(1):85-104 PMID: 5073353
The mechanism of muscular contraction.
Science. 1969 Jun 20;164(3886):1356-65 PMID: 4181952
Molecular control mechanisms in muscle contraction.
Physiol Rev. 1973 Jul;53(3):612-73 PMID: 4577547
Induced changes in orientation of the cross-bridges of glycerinated insect flight muscle.
Nature. 1965 Sep 18;207(5003):1276-80 PMID: 5884645
Evidence for a complex between myosin and ADP in relaxed muscle fibres.
Nat New Biol. 1972 Jan 5;235(53):23-4 PMID: 4259810
Identification of the transitory complex myosin-ATP by the use of , -methylene-ATP.
Nat New Biol. 1973 Feb 21;241(112):226-9 PMID: 4266989
Cooperation within actin filament in vertebrate skeletal muscle.
Nat New Biol. 1972 Jul 26;238(82):97-101 PMID: 4261616
The value of G degrees for the hydrolysis of ATP.
Biochim Biophys Acta. 1972 May 25;267(2):275-90 PMID: 4402900
Synthesis of ATP from ADP and inorganic phosphate at the myosin-subfragment 1 active site.
Eur J Biochem. 1974 Oct 1;48(1):287-95 PMID: 4375032
Individual states in the cycle of muscle contraction.
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2542-6 PMID: 4341699
Adenylyl imidodiphosphate, an adenosine triphosphate analog containing a P--N--P linkage.
Biochemistry. 1971 Jun 22;10(13):2484-9 PMID: 4326768
The reversibility of adenosine triphosphate cleavage by myosin.
Biochem J. 1973 Jun;133(2):323-8 PMID: 4269253
Elementary processes of the magnesium ion-dependent adenosine triphosphatase activity of heavy meromyosin. A transient kinetic approach to the study of kinases and adenosine triphosphatases and a colorimetric inorganic phosphate assay in situ.
Biochem J. 1972 Feb;126(3):635-44 PMID: 4263038
Transient state phosphate production in the hydrolysis of nucleoside triphosphates by myosin.
Biochemistry. 1970 Jul 21;9(15):2975-83 PMID: 4248809
Substructure of the myosin molecule. I. Subfragments of myosin by enzymic degradation.
J Mol Biol. 1969 May 28;42(1):1-29 PMID: 4241282
Mechanism of adenosine triphosphate hydrolysis by actomyosin.
Biochemistry. 1971 Dec 7;10(25):4617-24 PMID: 4258719
X-ray evidence for radial cross-bridge movement and for the sliding filament model in actively contracting skeletal muscle.
J Mol Biol. 1973 Jul 15;77(4):549-68 PMID: 4541885
General model of myosin filament structure. 3. Molecular packing arrangements in myosin filaments.
J Mol Biol. 1973 Jun 25;77(2):291-323 PMID: 4765362
P 1 ,P 5 -Di(adenosine-5')pentaphosphate, a potent multisubstrate inhibitor of adenylate kinase.
J Biol Chem. 1973 Feb 10;248(3):1121-3 PMID: 4734335
Myosin-product complex and its effect on the steady-state rate of nucleoside triphosphate hydrolysis.
Biochemistry. 1970 Jul 21;9(15):2984-91 PMID: 5474799
Evidence concerning crossbridge attachment during muscle contraction.
Nature. 1970 Jun 13;226(5250):1060-1 PMID: 5447020
Ultrastructure of insect flight muscle. I. Screw sense and structural grouping in the rigor cross-bridge lattice.
J Mol Biol. 1968 Jan 28;31(2):155-76 PMID: 5635532
The low-angle x-ray diagram of vertebrate striated muscle and its behaviour during contraction and rigor.
J Mol Biol. 1967 Dec 14;30(2):383-434 PMID: 5586931

Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1016/S0006-3495(75)85848-6

Published

1975-07-00

Pages

687-705

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1334729

Subset

External Links

PubMed source DOI: 10.1016/S0006-3495(75)85848-6 PMC Full Text View journal details

Analysis Services

Cross-bridge conformation as revealed by x-ray diffraction studies on insect flight muscles with ATP analogues.

Contact

WeChat Official Account