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PMID: 11340179 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Arabidopsis mutant cev1 has constitutively active jasmonate and ethylene signal pathways and enhanced resistance to pathogens.

The Plant cell ·Vol. 13 ·No. 5 ·2001-05-00 ·Pages 1025-33

Ellis C, Turner JG

Abstract

Jasmonates (JAs) inhibit plant growth and induce plant defense responses. To define genes in the Arabidopsis JA signal pathway, we screened for mutants with constitutive expression of a luciferase reporter for the JA-responsive promoter from the vegetative storage protein gene VSP1. One mutant, named constitutive expression of VSP1 (cev1), produced plants that were smaller than wild type, had stunted roots with long root hairs, accumulated anthocyanin, had constitutive expression of the defense-related genes VSP1, VSP2, Thi2.1, PDF1.2, and CHI-B, and had enhanced resistance to powdery mildew diseases. Genetic evidence indicated that the cev1 phenotype required both COI1, an essential component of the JA signal pathway, and ETR1, which encodes the ethylene receptor. We conclude that cev1 stimulates both the JA and the ethylene signal pathways and that CEV1 regulates an early step in an Arabidopsis defense pathway.

MeSH Terms
Arabidopsis/genetics,microbiology Arabidopsis Proteins Ascomycota Chitinases/biosynthesis Cyclopentanes/metabolism Defensins Ethylenes/metabolism Genes, Plant Luciferases Mutation Oxylipins Phenotype Plant Diseases/genetics Plant Growth Regulators/metabolism Plant Proteins/biosynthesis,metabolism Plant Roots/anatomy & histology Promoter Regions, Genetic Receptors, Cell Surface/metabolism Recombinant Fusion Proteins Signal Transduction/genetics Transgenes
Chemicals
Arabidopsis Proteins COI1 protein, Arabidopsis Cyclopentanes Defensins Ethylenes Oxylipins Plant Growth Regulators Plant Proteins Receptors, Cell Surface Recombinant Fusion Proteins ethylene receptors, plant jasmonic acid ethylene Luciferases Chitinases PLC-B protein, Phytolacca americana
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ellis C
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom.
Turner J G
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.13.5.1025
Published
2001-05-00
Pages
1025-33
Language
English
Region
England
NLM ID
9208688
PMCID
PMC135553
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.13.5.1025 PMC Full Text View journal details
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