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PMID: 11316880 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structural basis of pheromone binding to mouse major urinary protein (MUP-I).

Protein science : a publication of the Protein Society ·Vol. 10 ·No. 5 ·2001-05-00 ·Pages 997-1004

Timm DE, Baker LJ, Mueller H, Zidek L, Novotny MV

Abstract

The mouse major urinary proteins are pheromone-binding proteins that function as carriers of volatile effectors of mouse physiology and behavior. Crystal structures of recombinant mouse major urinary protein-I (MUP-I) complexed with the synthetic pheromones, 2-sec-butyl-4,5-dihydrothiazole and 6-hydroxy-6-methyl-3-heptanone, have been determined at high resolution. The purification of MUP-I from mouse liver and a high-resolution structure of the natural isolate are also reported. These results show the binding of 6-hydroxy-6-methyl-3-heptanone to MUP-I, unambiguously define ligand orientations for two pheromones within the MUP-I binding site, and suggest how different chemical classes of pheromones can be accommodated within the MUP-I beta-barrel.

MeSH Terms
Animals Binding Sites Crystallography, X-Ray Hydrogen Bonding Liver/chemistry Mice Models, Molecular Pheromones/chemical synthesis,chemistry,metabolism Protein Binding Protein Structure, Secondary Proteins/chemistry,metabolism Structure-Activity Relationship
Chemicals
Pheromones Proteins major urinary proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Timm D E
Department of Biochemistry, Indiana University, Indianapolis, Indiana 46202, USA. dtimm@iupui.edu
Baker L J
Mueller H
Zidek L
Novotny M V
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Article Info
Journal
Protein science : a publication of the Protein Society
Abbr.
Protein Sci
ISSN
0961-8368
DOI
10.1110/ps.52201
Email
dtimm@iupui.edu
Published
2001-05-00
Pages
997-1004
Language
English
Region
United States
NLM ID
9211750
PMCID
PMC2374202
Subset
IM
Grants
NIGMS NIH HHS · R01 GM055055 · United States
NIGMS NIH HHS · GM55055 · United States
NIDCD NIH HHS · DC02418 · United States
NIDDK NIH HHS · DK54738 · United States
NIGMS NIH HHS · R29 GM055055 · United States
Databases
PDB
1IO4, 1IO5, 1IO6
External Links
PubMed source DOI: 10.1110/ps.52201 PMC Full Text View journal details
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